VP 404A - Blot Type Replicators Datasheet

9823 Pacific Heights Boulevard, Suite T, San Diego, CA 92121 (858) 455-0643 ? Fax: (858) 455-0703 ? email: sales@vp-scientific.com TECHNICAL NOTE 137 TIPS ON THE CARE AND USE OF BOLT REPLICATORS Disinfecting: The Replicators can be sterilized by hot air oven, autoclaving, treating in 10% bleach or alcohol flaming. The pins can be cleaned between source plates by dipping briefly in a 10% bleach solution, followed by a series of two sterile dH2O baths (all in tip lid boxes), then a 99% isopropanol bath in a non flammable VP 420 glass reservoir. The pins can be air dried, using a hair drier or by flaming the isopropanol. Between baths, remove the liquid from the pin tips by blotting on a lint-free blotting paper (VP 522) as lint from paper towels will interfere with uniform liquid transfers. This blotting step is also very important to reduce carry over. If you flame, do not blot the isopropanol before igniting and be prepared for a "whoosh" upon ignition. Keep the isopropanol reservoir and blotting paper at least 3 feet from the flame. We strongly advise you to use a non-flammable alcohol reservoir such as the VP 420 Pyrex® alcohol reservoir to avoid laboratory fires. If you don't flame, blot on the lint-free blotting paper and let the isopropanol evaporate. The evaporation can be speeded up with a hair dryer. It is important that the pins be dry before going into the next source plate. ** Flaming just entails igniting the alcohol on the pins and NOT heating the pins directly in a Bunsen burner which can damage the corrosion resistant properties of the stainless steel pins. Care: Before each day’s use we recommend that the pins be cleaned with VP 110 Pin Cleaning Solution, which is designed to clean stainless steel and condition the pins. If the pins should be coated with organic material they can be mechanically cleaned with the VP 425 brush and Ivory dish detergent. If you have access to an ultrasonic bath, we recommend using MICRO 90®, from Cole Parmer®, at a 1/100 dilution in the ultrasonic bath. If you use an ultrasonic bath, hold the Replicator in the bath without letting the pins touch the bottom of the reservoir (the vibrating bottom surface of the sonicator's reservoir may damage the pin tips). The Cole Parmer Catalog #P-08857-02 Ultrasonic Cleaner is ideal for cleaning the Replicators. It is not necessary to clean the replicators in an ultrasonic cleaner if you clean the pins with bleach and brush with detergent after each day's use.