Wakopak Fluofix- Brochure

HICHROMChromatography Columns and SuppliesHichrom Limited1 The Markham Centre, Station RoadTheale, Reading, Berks, RG7 4PE, UKTel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484Email: sales@hichrom.co.uk www.hichrom.co.ukCatalogue 9LC COLUMNSWakopak FluofixLC Columns – Wakopak Fluofixwww.hichrom.co.uk • sales@hichrom.co.uk • Tel: 0118 930 3660 261Hichrom LimitedWakopak FluofixWAKOPAK® FLUOFIX®Fluofix® is a fluorinated silica based material for selective reversed-phase HPLC. Initially manufactured by Neos, it is now manufactured by Wako Chemicals, Japan. The newer Wakopak Fluofix-ll 120E is now the recommended Fluofix phase for method development. • Unique selectivity• High recognition capability of halogenated compounds• Separation of geometric and positional isomers• High durabilityWakopak Phase Particle Size (µm) Pore Size (Å) Surface Area (m2/g) Endcapped ApplicationFluofix-ll 120E 5 120 300 Yes General analysesFluofix 120E 5 120 300 Yes General analysesFluofix 120N 5 120 300 No Acidic compoundsWakopak Fluofix phases are bonded with branched chain perfluorohexyl groups on 120Å pore size silica (see Figure 1). Both endcapped and non- endcapped versions are available. They exhibit increased retention and selectivity of compounds containing fluorine and chlorine substituents when compared to C18 phases. This is due to the more polar nature of the carbon-fluorine bond in Fluofix compared to the carbon-hydrogen bond of C18 phases. Wakopak Fluofix-ll 120E has improved endcapping efficiency, in order to minimise non-specific adsorption compared with Fluofix 120E. The retention capacity of Fluofix-ll 120E is comparable to that of a C4 bonded phase. Figure 2 shows a comparison of the separation of fluorinated positional isomers using Wakopak Fluofix-ll 120E, Wakopak Fluofix 120E and ODS (C18) columns. The high shape selectivity observed with Fluofix-ll 120E and Fluofix 120E for halogenated isomers, is due to the extra rigidity imposed by the perfluorinated bonded phase. Wakopak Fluofix phases are also highly selective for the analysis of non-halogenated polar compounds containing hydroxyl, carboxyl, nitro and other polar groups. This is most apparent when the functional groups are located on an aromatic or other rigid ring system. Figure 1. Schematic surface model of FluofixOrdering InformationWakopak PhaseColumn Dimensions1,2 (mm)50 x 2.0 150 x 2.0 250 x 2.0 30 x 4.6 50 x 4.6 150 x 4.6 250 x 4.6Fluofix-ll 120E£333 233-63393£432 236-63403£500 233-63413£291 237-63433£333 230-63423£432 239-63373£500 236-63383Fluofix 120E Fluofix 120N£416 236-61943 230-61843£475 233-61953 237-61853---£308 237-61973 231-61873£391 234-61983 238-61883£475 231-61993 235-61893£591 238-62003 238-619031 10mm i.d. columns also available 2 Part numbers listed correspond to columns with Waters type endfittings. Columns with DuPont type endfittings also available – please enquireWakopak Wakosil columns also available – please enquire for details.Column dimensions: 250 x 4.6mmEluent: CH3OH - H2O (60:40)Flow rate: 1ml/minColumn temperature: 40°CFigure 2. Fluorinated positional isomers*1. Uracil2. Benzene3. Fluorobenzene4. p-Difluorobenzene5. o-Difluorobenzene6. m-Difluorobenzene7. 1,3,5-Trifluorobenzene8. 1,2,3,4-Tetrafluorobenzene9. Pentafluorobenzene10. HexafluorobenzeneFluofix-ll 120EFluofix 120EODS Column0 10 20 30 Mins1243567810912435,67810914 2,35678109*The comparative separations presented here may not be representative for all applications.