LIVE WEBINAR: Polar Compound Retention Part II - September 12!
Join us for Part 2 of this free and informative live webinar!
The successful retention and quantification of polar analytes and metabolites continues to be an exceptional chromatographic challenge for analytical scientists. Hydrophilic interaction chromatography (HILIC) can be used as a chromatographic technique to successfully improve the retention of very polar species while improving sensitivity in mass spectrometry, shortening sample handling steps and provide orthogonal selectivity compared to RPLC. Due to the complex, multi-modal retention mechanisms present in HILIC, predicting retention behavior to develop HILIC separations can be rather challenging. Further difficulties arise due to the altered mobile phase pH and analyte pKa values in highly organic HILIC solvents.
This seminar is intended to give the chromatographer several tips and tricks for retaining and separating polar compounds using HILIC separation techniques. A majority of the presentation will be spent on developing best practices for successfully developing robust and reproducible HILIC methods.
You will Learn:
- History and development of HILIC stationary phases
- How to take better advantage of HILIC to provide complementary data
- Tips and Tricks for the best results using HILIC
- Proper usage and considerations for column lifetime
Who should attend:
This informative seminar is designed for chromatographers who analyze polar compounds using reversed-phase and HILIC techniques and wish to learn and improve the retention and separation of this challenging class of compounds.
Tuesday, September 12, 2017
1:30 pm - 2:30 pm ET
(12:30pm CT, 11:30am MT, 10:30am PT)
Learn more or register here.