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MedChemExpress - Model Fura-2 AM -108964-32-5

Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ (calcium ion) indicator.

Fura-2 AM

MCE China：Fura-2 AM

Brand：MedChemExpress (MCE)

Cat. No.HY-101897

CAS：108964-32-5

Synonyms：Fura-2 Acetoxymethyl ester

Purity：99%

Storage：-20°C, protect from light *The compound is unstable in solutions, freshly prepared is recommended.

Shipping：Room temperature in continental US; may vary elsewhere.

Description：Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ (calcium ion) indicator.

In Vitro：Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.1. First, prepare the 1 mM Fura-2 AM stock by adding 50 μL of DMSO to a 50 μg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 μL of Fura-2 AM stock to generate a 1μM Fura-2 AM solution. Vortex the solution vigorously for 1 min.3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.6. Mount the coverslip on the imaging chamber.

Emission (Em)：505

Excitation (Ex)：336

References：

[1]. Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23):1067. [Content Brief]

Brand introduction:
•   MCE (MedChemExpress) has a global exclusive compound library of more than 200 kinds, and we are committed to providing the most comprehensive range of high-quality small molecule active compounds for scientific research customers around the world；
•   More than 50,000 highly selective inhibitors and agonists are involved in various popular signaling pathways and disease areas；
•   The products cover a variety of recombinant proteins, peptides, commonly used kits, more PROTAC, ADC and other characteristic products, widely used in new drug research and development, life science and other scientific research projects；
•   Provide virtual screening, ion channel screening, metabolomics analysis detection analysis, drug screening and other professional technical services；
•   It has a professional experimental center and strict quality control and verification system；
•   Provide LC/MS, NMR, HPLC, chiral analysis, elemental analysis and other quality inspection reports to ensure the high purity and high quality of products；
•   The biological activity of the products has been verified by the experiments of customers in various countries；
•   A variety of top journals such as Nature, Cell, Science and pharmaceutical patents have included the scientific research results of MCE customers；
•   Our professional team tracks the latest pharmaceutical and life science research and provides you with the latest active compounds in the world；
•   It has established long-term cooperation with the world's major pharmaceutical companies and well-known scientific research institutions。

MedChemExpress - Model Fura-2 AM -108964-32-5

MCE products for research use only. We do not sell...

Fura-2 AM