MedChemExpress - Model Elastase, Porcine pancreas -39445-21-1
Elastase, Porcine pancreas (EC 3.4.21.36) is a single polypeptide chain of 240 amino acid residues, derived from pig pancreas. Elastase, Porcine pancreas is a serine protease that can hydrolyze proteins and polypeptide. Elastase from porcine pancreas can induce emphysema in hamsters[1][2][3].MCE products for research use only. We do not sell to patients.
Elastase, Porcine pancreas
MCE China：Elastase, Porcine pancreas
Brand：MedChemExpress (MCE)
Cat. No.HY-P2974
CAS：39445-21-1
Synonyms：EC 3.4.21.36; Pancreatopeptidase E
Storage：Please store the product under the recommended conditions in the Certificate of Analysis.
Shipping：Room temperature in continental US; may vary elsewhere.
Description：Elastase, Porcine pancreas (EC 3.4.21.36) is a single polypeptide chain of 240 amino acid residues, derived from pig pancreas. Elastase, Porcine pancreas is a serine protease that can hydrolyze proteins and polypeptide. Elastase from porcine pancreas can induce emphysema in hamsters.
In Vitro：In-Solution Digestion Protocol1. Resuspend Elastase in double-distilled water to a final concentration of 1 mg/mL. Store reconstituted Elastase at 4°C for up to 2 weeks.2. Resuspend the protein in reaction buffer.3. Add Elastase to protein solution; mix. We recommended using enzyme:protein ratios of 1:20 to 1:100. 4. Incubate 2-18 hours at 37°C. 5. Stop the reaction by adding 10% formic acid or TFA to a final concentration of 0.5% or by heating at 95°C for 10 minutes.
In Vivo：Elastase from porcine pancreas induces emphysema in hamsters[3].
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References：
[1]. Shotton DM, et, al. Amino-acid sequence of porcine pancreatic elastase and its homologies with other serine proteinases. Nature. 1970 Feb 28;225(5235):802-6. [Content Brief]
[2]. Teshima T, et, al. A new class of heterocyclic serine protease inhibitors. Inhibition of human leukocyte elastase, porcine pancreatic elastase, cathepsin G, and bovine chymotrypsin A alpha with substituted benzoxazinones, quinazolines, and anthranilates. J Biol Chem. 1982 May 10;257(9):5085-91. [Content Brief]
[3]. Stone PJ, et, al. Induction and exacerbation of emphysema in hamsters with human neutrophil elastase inactivated reversibly by a peptide boronic acid. Am Rev Respir Dis. 1990 Jan;141(1):47-52. [Content Brief]
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