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taizyModel tz -500L yogurt fermentation tank

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  Yogurt fermentation is a complex aerobic (or anaerobic) complex biochemical process that requires pure air immersion culture with sterile air and culture medium. Systems engineering for sterilization, preparation of sterile air, control of fermentation processes and preparation of process pipelines.      The performance of the yogurt fermentation tank can be divided into the following aspects: easy operation of feeding and discharging, safety to maintain the sterility, guaranteeing the safety of the tank, improvement of system control ability, and most importantly, improvement of dissolved oxygen ability .
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1.Passivation treatment of tank (anti-corrosion)

      The passivation treatment of the tank is to improve the corrosion resistance of the tank, especially for the fermentation system in which there are more Cl- and more in the microalgae fermentation process, and the tank that has been used for a long time also needs to be processed. For details on the modification of this performance, please refer to the previous issue of the public account "Fermentation Engineering":

Fermentation equipment: stainless steel passivation process to protect the internal and external surfaces of the fermentation tank

2. Pretreatment of special materials (liquid distribution system)

     Viscous materials can be introduced into the tank using a homogenizer or a homogenizer pump. Glucose can better increase sugar bowl unit consumption.

3. Filling pipeline in the tank (feeding system)

      For volatile flow-feeding projects, if continuous low-speed flow-feeding is adopted, the flow-feeding pipes are better modified to be inserted below the liquid level to prevent air loss and material loss and process instability. effect

4. Multi-head feeding device (feeding system, removable and refittable)

     For the complex feeding process, sometimes the tank needs to be temporarily added with a feeding inlet. If it is a small test, the multi-fast connector method can be retrofitted. After the process is fixed, a hole can be added to increase the quick connector.

5. Add electrode socket (detection)

     There are three electrodes connected to the yogurt fermentation tank, namely: pH, DO, and T, which are sufficient for conventional fermentation, but for some special types of fermentation, the electrode socket is missing, such as methanol induction Type fermentation, you may need to increase the methanol membrane permeator, and then the methanol feed pump online coupling control, you only need to set the point concentration, there is no need to sample the methanol concentration and then intermittent feed or continuous flow speed adjustment, so For large-scale production, we can consider improving the tank, adding electrode sockets, and improving production efficiency.

6. Temporarily change the sampling port (sampling, electrode socket, detachable modification)

     For small tanks without bottom valve and intermediate sampling port, during long-term fermentation, sampling may be difficult in the middle and late stages. If you modify the unused electrode socket according to the following figure, this problem can be solved. This solution is simple It is feasible, the sampling loss is small and removable, and the advantages are obvious.

7, increase the bottom valve (discharging, cleaning)

     Some tank designs have defects. As shown in the figure below, there is no bottom valve, and the drainage and discharge are driven by pressurized air. This equipment is not clean and the discharge of the tank is not complete. It is necessary to modify and increase the bottom valve. Yes.

8. Use of cyclone separator (escape liquid, defoaming)

     The function of the whirlwind is to eliminate foam, limit escape, and avoid the toxic effect of chemical defoaming agents on bacteria, and its efficiency is more efficient than the physical defoaming solution. For details, please refer to the public issue of the "Fermentation Engineering" historical release: fermentation Equipment: Cyclone separator controlled by escaping of fermentation broth

9. Stirring paddle improvement (dissolved oxygen)

     The stirrer installed in the fermentation tank is first used to disperse the air bubbles to obtain the highest mass transfer coefficient KLa as possible. In addition, the agitated fermentation broth should be circulated to increase the average residence time of air bubbles, and evenly distributed throughout the system to prevent them from agglomerating. Earlier in mechanical stirring fermentation tanks were usually equipped with several radial disc turbine stirrers, but it was easy to stratify the medium being stirred to form several zones, thus forming an oxygen partial pressure gradient between the lower and upper parts of the tank, leading to the tank Difference in KLa values ​​between the upper and lower parts. Recently, the stirring system of the fermentation tank mostly uses a turbo agitator at the bottom of the tank to disperse the air, and a set of axial flow agitators is installed on the bottom to circulate the culture medium, evenly distribute air bubbles, strengthen heat transfer and eliminate the tank. Oxygen gradient between the upper and lower parts.

Stirring leaf form:

(1) Open-turbine stirring blade with disc—high turbulence, radial flow.

(2) Pitched blade (pitched blade) turbine (p-4) — 45 ° four-blade, axial flow.

(3) Reversingpitch Stirring leaves—two up, two down, radial flow.

(4) High-efficiency axial-flow stirring blade --- A310, axial flow.

(5) Mixed flow stirring blades-A315, axial flow, a small amount of radial flow.

(6) Concaveblade radial —CD-6, radial flow.

Participate in the specific historical release of the public account "Fermentation Engineering" in the past period:

Fermentation equipment: selection criteria of stirrers and the effect of different leaf shapes of the stirrer on the fermented mash

10. Improvement of air sterilization ability (miscellaneous bacteria, phage)

     Bacteriophages are extremely small but also easily thermally denatured. They can easily enter the tank through the air system. If bacterial fermentation is performed, it is recommended to set a heating and sterilization link in the fermentation air system. It is more economical to use an air compressor to compress the heat. Heat preservation and sterilization, inlet temperature is 21-22 ℃, outlet temperature is 187-189 ℃, pressure is 0.7 Mpa.

11. Heat and energy transfer system (defect improvement)

     Once there was a veterinary drug fermentation company in Gansu, there was no heating water inlet device in the expansion tank. After the tank was transferred, it was planned to rely on microbial heat to maintain the energy of the system. However, in winter, this solution would not work, so the reformation of the energy system Strengthening is also a priority that every fermentation engineer should consider.

12.Stirring power

     This item involves the selection of the stirring motor. The speed of the motor is not the speed of the stirring shaft. There is also a speed reducer between the motor and the stirring shaft. It involves speed ratio conversion. If the motor power is too small, it will

Affecting the ability to dissolve oxygen, according to the characteristics of the microorganisms in your hand, it is a good choice to replace the motor with a higher speed or speed ratio in a timely manner.

13.Air distributor (DO)

     The ratio of diameter to height of small tanks is not as good as that of large tanks. After entering the tank, it moves to the liquid level quickly. To dissolve oxygen, an air distributor can be added to the end of the inlet pipe (some small tank manufacturers do not make distributors). You need to pay attention to the nature of the fermentation broth , Too sticky may block the small holes of the air distributor.