Gentronix - 96-well MNT

Ames assays are bacterial reverse mutation assays that use a variety of Salmonella (or E. coli) strains to detect a range of point mutations and have high sensitivity when used in combination. The Ames test is the most established assay for detecting direct-acting mutagens and forms a key aspect of regulatory genotoxicity testing across industries. As such, an early stage prediction of Ames test outcome is a key step in assessing the toxicological hazard of test substances during development. Gentronix offers a range of service options to meet your project requirements.

MNT 96-well - Test Cell Line: TK6
Metabolic activation: Typically, induced rat liver S9, though other sources can also be used
Short +S9
Long –S9
2 arm study
Typical test item requirements: 20 mg (ICH S2(R1)) – < 100 mg (OECD 487)
+ FISH: < 30 mg
Study Duration: < 4 Weeks (TK6 MNT)
< 6 Weeks (TK6 MNT + FISH)
Endpoint: Micronucleus Formation / Mode of Action