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Alpha Synuclein Protein
Product Name: Alpha Synuclein Protein. Description: . Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 1). Applications: WB, SDS-PAGE, In vivo assay, In vitro assay. Concentration: Lot/batch specific. See included datasheet.. Conjugates: No tag. Nature: Recombinant. Species: Human. Expression System: E. coli. Amino Acid Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA. Purity: >95%. Protein Length: Full Length.
Biological Activity: Endogenous alpha-synuclein phosphorylation. 100 µM alpha synuclein protein monomer (SPR-321) seeded with 10 uM alpha synuclein protein PFF (SPR-322) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Field Of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Storage Buffer: PBS
Storage Temperature: -80ºC
Shipping Temperature: Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Purification: Ion-exchange Purified
Cite This Product: Human Recombinant Alpha Synuclein Protein (StressMarq Biosciences, Canada, Cat # SPR-322)
Certificate Of Analysis: Certified >95% pure using SDS-PAGE analysis.
Other Relevant Information: For best results, sonicate immediately prior to use. Refer to the Neurodegenerative Protein Handling Instructions on our website, or on the product datasheet for further information..
Alternative Names: Active Alpha synuclein PFFs, Active Alpha synuclein aggregates, Alpha synuclein PFF, Active Alpha synuclein protein aggregates, Active Alpha synuclein aggregates, Active Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkinson`s disease familial 1 Protein, Active Alpha synuclein protein seed
Research Areas: Alzheimer`s Disease, Neurodegeneration, Neuroscience, Parkinson`s Disease, Synuclein, Tangles & Tau, Multiple System Atrophy
Cellular Localization: Cytoplasm, Membrane, Nucleus
Accession Number: NP_000336.1
Gene ID: 6622
Swiss Prot: P37840
Scientific Background: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson`s disease. Parkinson`s disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
References:
1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).
2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.
3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.
4. Kokhan V.S., Afanasyeva M.A., Van`kin G. (2012) Behav. Brain. Res. 231(1): 226-230.
5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.
6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.