Andor - Model Dragonfly - Microscopy System
From Andor - Microscopy
The game-changer in confocal microscopy - with the Andor Dragonfly you can image at an unrivalled, multi-modal combination of speed, sensitivity and confocality. The Dragonfly naturally benefits from Andor EMCCD and sCMOS technologies, with motorized optical zoom, the system delivers industry-leading signal to noise and image fidelity. Applications ranging from single molecule to live cell confocal, TIRFM to whole embryo and thick tissue imaging all benefit from the system’s speed and sensitivity. Dragonfly delivers real-time visualization for rapid specimen evaluation and GPU-accelerated deconvolution to maximize resolution and throughput.
- Instant Confocal
- Simultaneous multi-color TIRF
- Laser widefield imaging
- Single molecule imaging
- VIS - NIR wavelengths
The Dragonfly offers you a sharp insight into the world of your specimen by employing a novel multi-point confocal system, optimized for fast, flexible and sensitive scanning. It extends your sample’s longevity and significantly speeds up data acquisition.
As well as confocal, the system provides further extraordinary control of illumination by offering enhanced widefield and TIRF microscopy, extending the reach from nm to mm and from single molecules to cells, tissues, organoids and embryos.
Traditional Point Scanning Confocal
Dragonfly Confocal Imaging System
Andor Dragonfly features multiple innovative optical solutions to create the best possible combination of light uniformity, throughput, resolution and power in a confocal microscopy system.
Borealis Uniform Illumination
Uniformity of illumination plays a significant role in obtaining seamless image tiling and more accurate cross-field analysis.
With Borealis, and its unique Perfect Illumination Delivery system, you can ensure that the edges and corners of your sample never suffer from non-uniformities.
A unique, user-defined, 3-positon zoom in the camera port makes it possible to match the camera pixel size to an objective lens.
This ensures that the Nyquist sampling criterion is met and the specimen is sufficiently oversampled for the best possible spatial resolution.
For certain applications, such as Ca2+ imaging or localization microscopy, a higher power density per unit area may be useful.
In these circumstances, obtaining a high temporal resolution is paramount, while photo-bleaching is not critically endangering the sample during such a time lapse.
Confocal, widefield and TIRFM modes for your imaging experiment!
With three modalities at your command, you can explore the in vitro and in vivo biology of many different specimens, selecting the best model organism and modality to suit your needs. Imaging everything from organoids and deep neural structures along the z-dimension to yeast, bacteria and single molecules requires flexibility of imaging mode, magnification and illumination. This level of control preserves specimens and trades speed and longevity in dynamic and large scale imaging tasks.
At the heart of the Dragonfly Imaging Platform is a newly designed microlens confocal scanner, tightly integrated with our highest sensitivity cameras and presented via our new imaging software, Fusion. The result is speeds of 10 to 20 times faster than a traditional confocal, leaving you with no more waiting for an image to be built up point-by-point and line-by-line. Dragonfly offers the following major benefits:
- Minimal phototoxicity and photobleaching - ideal for live or delicate specimens.
- High speeds for imaging fast dynamic events, or high throughput.
- Large uniform field of view for larger specimens or montage stitching.
Central to the Dragonfly concept is the goal of maximizing throughput, which is achieved by optimizing both imaging performance and data flow. Fusion and Imaris provide seamless transitions from imaging to deconvolution, visualization to analysis. As a result you spend more time collecting high quality data and less time pre-processing and transferring between systems.
Speed Hypothesis Testing
Hypothesis testing is a fundamental part of the scientific method. The familiar cycle of data creation and evaluation are delivered by the Dragonfly workflow, supporting demanding experimental regimes. Extended observations, large fields of view and high quality data provide the input for powerful statistical analysis and data mining.
Fusion is the result of over 100 person-years of shared imaging and development experience, making it easy to design an experiment, choose necessary devices and configure optimal data acquisition protocols. It simplifies the control of the Dragonfly system, translating imaging modes into software states and fluorophores into channels to help you build protocols in just a few mouse clicks.
Integrating deconvolution is simple, tasking the GPU with the associated calculations so that acquisition continues unaffected: a big time saver! Results are delivered with your data, all in one software package. Real-time 3D visualization, split channel view and MIP are fully configurable during acquisition to let you view the data in a mode which best suits your experiment.
Real-time rendering in Fusion allows for an on-going verification of the quality of your experiment and the status of your sample.
Having a near-instantaneous feedback on the 3D properties of the specimen is critical for evaluation of experimental parameters including signal to noise, illumination power and sample viability, its position in 3D and in relation to its cellular or tissue neighbours. Render quality and speed can be rapidly adjusted following any change in your experiment and can be easily accessed in the Fusion software control panel.
All the necessary functionality for data management, visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets with Imaris
Visualize Complex Data
Welcome to a new era of data interpretation, plotting and presentation. ImarisVantage enables you to compare and contrast experimental groups by visualizing your image data in five dimensions as uni- or multi-variate scatterplots. Along with the use of box + whisker plots Vantage will help you interpret intrinsically complex and dynamic phenomena.
ImarisVantage allows researchers to dissect their multi-dimensional, multi-object images by creating a series of fully customizable plots for better understanding of hidden relationships and associations between calculated measurements, objects or groups of objects.
FilamentTracer is the most advanced software product for the automatic detection of neurons (dendritic trees, axons and spines), microtubules, and other filament-like structures in 2D, 3D and 4D.
When combined with ImarisTrack, detection of temporal changes in length and volume of developing spines and dendrites will help researchers understand alterations caused by developmental and environmental changes.
Analyse and Understand Cell Fate
ImarisLineage is the new module which builds on the functionality of the powerful and widely used ImarisTrack. ImarisLineage provides tools for accurate automated or manual tracking of dividing cells in 2D/3D time series.
ImarisLineage enables creation of an interactive lineage tree where the cell fate can be traced from one progenitor cell to the final generation.