Alexa Fluor - Model 647 -Anti-Mouse IgD Antibody

Isotype Control: Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)

Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections^2,3.

Application References

(PubMed link indicates BioLegend citation)

1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed

Product Citations

1. Barwick BG, et al. 2018. Nat Commun. 9:1900. PubMed
2. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
3. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
4. Adori M, et al. 2018. J Immunol. 200:775. PubMed
5. Avery DT, et al. 2018. J Exp Med. 215:2073. PubMed
6. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
7. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
8. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
9. Cheng Q, et al. 2018. PLoS Pathog. 14:e1007440. PubMed
10. Shi B, et al. 2018. J Immunol. 200:586. PubMed
11. Vacchio MS, et al. 2020. Immunity. 51(3):465-478. PubMed
12. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed

See MoreRRIDAB_893529 (BioLegend Cat. No. 405707)
AB_893528 (BioLegend Cat. No. 405708)

Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797 View all products for this Gene ID

UniProt: View information about IgD on UniProt.org