Transcreener - Model AMP2/GMP2 -Assay Kit - TR-FRET Readout
The Transcreener® AMP2/GMP2 Assay kit with TR-FRET readout uses displacement of a tracer by AMP/GMP to measure enzymatic activity. This disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering. The assay produces an excellent signal at low substrate conversions for a broad range of initial substrate concentrations (0.1-1,000 µM). Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs. The universal detection method allows screening of virtually any AMP/GMP-producing enzyme in a high throughput format.
- Direct detection of unlabeled AMP/GMP
- Robust Assay Z` > 0.7 under initial velocity conditions
- Far-red fluorescent readouts minimize compound interference
- A safe, non-radioactive method
- Universal - use with virtually any AMP/GMP-producing enzyme
- Measure enzymatic activity of AMP/GMP-producing enzymes
- Screen compound libraries for enzyme modulators
- Quantify inhibitor potency
- Inhibitor selectivity profiling
- Measure drug-target residence time
Along with PDEs the Phosphodiesterase Assay kit can be used with:
Run your enzyme reaction, add Transcreener reagents, and read your plates. The AMP/GMP assay kit is compatible with 96, 384, and 1536-well formats.
- 384-Well Format
- Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
- Z’ specifications to be met using Transcreener ADP2 Assay reagents
- Read time to achieve Z’ specifications ≤ 5 minutes
Look for these stickers on instruments that have successfully met the validation criteria.
The instrument validation program is a collaborative effort with the major suppliers of multimode readers to ensure that Transcreener assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance. We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.