AssayLite - Fluorescent Singleplex Kits

I. Competition Fluorescent Immunoassay Principles.

1. Step 1: A specific antibody coated on the plate uniformly and standard and/or sample along with fluorescent labeled protein are added to the well.
2. Step 2: Fluorescent labeled protein compete with none labeled protein binding to the antibody. The blank has the highest fluorescent signal and the signal decreases when the non-labeled protein concentration increases.

II. Sandwich Fluorescent Immunoassay Principles.

1. Step 1: A specific antibody coated on the plate uniformly and standard and/or sample are added to the well.
2. Step 2: Targeted antibody bind to the standard and/or sample.
3. Step 3: Antibody labeled with fluorescent probe is added to the well.
4. Step 4: Antibody with fluorescent probe binds to the targeted protein and the specific fluorescent signal is read by fluorescent plate reader.

• Easy to use with less assay time
• Superior accuracy and reproducibility
• Wider linearity range
• Very competitively priced
• Fast and friendly customer service