autoPulse® - TFF System for High-Throughput Sample Concentration and Diafiltration

Key Features:

• High-Throughput
  Process up to 54 samples per run with up to 4 processed in parallel.
• Low Hold-Up Volume
  Final volume as low as 250 µL with ±25 µL resolution.
• High-Flux Performance
  Dual-diaphragm pumps enable permeate flow up to 1.7× faster than µPulse and 4× faster than centrifugal filters.
• Buffer Flexibility
  Use up to 4 external buffer reservoirs or on-deck conical tubes.
• Automatic Cleaning-in-Place
  Automatically cleans the liquid path and performs buffer/water rinsing for optimal sample conditions.
• Disposable Fluid Path
  Re-engineered disposable filter chips with integrated transfer tubing eliminate cross-contamination.
• Remote Monitoring
  Intuitive web-based software allows full protocol setup, remote monitoring, and run log exporting.

Run Versatile Samples:

• Nanoparticle Processing
  Efficiently process lipid nanoparticles, liposomes, and polymeric nanoparticles for drug delivery and therapeutic efficacy.
• Protein Processing
  Optimize protein preparation with fast and gentle processes including concentration, formulation, desalting, and refolding.
• Bioconjugate Cleanup
  Ensure gentle and efficient removal of small unconjugated molecules from crude biomolecular labeling reactions.
• Nucleic Acid Processing
  Simplify in vitro synthesis of RNA and linear or plasmid DNA with efficient concentration and buffer exchange.
• Cell & Particle Harvesting
  Streamline the harvesting of cells, extracellular vesicles, enzymes, and Virus-Like Particles (VLPs) with high yield and quality.
• Virus Processing
  Gently concentrate and exchange buffers for AAVs, bacteriophages, and lentiviruses while preserving structural integrity.
• Vaccine Development
  Optimize DNA, RNA, and polysaccharide vaccine formulations for stable, cost-efficient results.

Membrane Specifications

Available membrane materials and MWCOs sizes: mPES: 5, 10, 30, 50, 100 and 300 kDA
RC: 5, 10, 30 and 100 kDA
Membrane area: 3.5-12 cm2
Membrane Storage: 40% Glycerol, 0.004% Thiomersal

System Specifications:

Recommended Starting Volume: 1 mL - 100 mL
Minimum Final Volume: 250 µL
Suggested Max. Sample Viscosity: 100 cP
SBS format labware
Buffer Reservoirs: 50mL conicals on deck or external buffer reservoirs
External permeate collection with overflow monitoring
Data management meets 21 CFR Part 11 standards for GMP
 

Electrical Specifications:

110 V - 240 V, 50 Hz - 60 Hz, 50 W typical, 150 W max. All outlet types are supported
Connectivity: WiFi/Ethernet 
 

Dimensions: 

Height: 830 mm (32.7”)
Height: 1070 mm (42.2”) (with door open)
Width: 835 mm (32.9”)
Depth: 740 mm (29.1”)
Weight: 81 kg (178.6 lbs)