Aviva Systems Biology - Model MMP9 (OKEH00522) -ELISA Kit (Rat)
Aviva Systems Biology MMP9 ELISA Kit (Rat) (OKEH00522) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Rat MMP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat MMP9 captured in well.
- Predicted Species Reactivity Rat
- ELISA Kit Detection Method Colorimetric, OD450 nm
- ELISA Kit Duration ~ 3 Hours
- ELISA Kit Range 0.78 - 50ng/mL
- ELISA Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
- ELISA Kit Reproducibility Mean Intra-assay CV%: < 4.2% (n = 20)
- Mean Inter-assay CV%: < 7.4% (n = 20)
- Reconstitution and Storage : Store as indicated in product manual.
- Sample Type : Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
- Sensitivity : 0.433 ng/mL
- Specificity : Natural and recombinant Rat Matrix metalloproteinase-9
- Assay Info : Assay Methodology: Quantitative Sandwich ELISA