Model AxL CTLS -Axially Swept Light Sheet Microscopy System
The Axially Swept Light Sheet Microscopy System, known as AxL CTLS, offers advanced imaging capabilities for cleared specimen visualization. Designed with a fully automated macro zoom microscope and high numerical aperture (NA) apochromatic objectives, it features dual-sided light sheet illumination for comprehensive imaging of whole organs and small animals. This system employs a patented axially swept light sheet microscopy (ASLM) technique to generate a thin, long, and uniform lightsheet, bolstered by custom-designed excitation objectives. Capable of imaging one cubic centimeter in under a minute, it utilizes a back-thinned sCMOS camera. The AxL CTLS system includes a compact scanner with dual-sided illumination optics and a robust sCMOS 2304 x 2304 pixel camera, ensuring a 95% quantum efficiency for precise imaging. It features high-speed axial chromatic focus adjustment, motorized zoom, and an electrically tunable lens. Integrated with SlideBook software, it expertly manages data for high-resolution imaging tasks.
AxL Cleared Tissue LightSheet (AxL CTLS) is a fully automated macro zoom microscope with high NA apochromatic objectives and dual-sided light sheet illumination for imaging whole organs to small animals. Custom-designed excitation objectives and patented axially swept light sheet microscopy (ASLM) produce an exceptionally thin, long and uniform lightsheet for large-scale high-resolution imaging. Using a back-thinned sCMOS camera, AxL CTLS images one cubic centimeter in less than a minute.
Axially swept lightsheet microscopy (ASLM) – as published in Dean, Fiolka et al., 2015 – scans the light sheet in its propagation direction using high-speed remote focusing synchronized to the rolling shutter readout of an sCMOS camera at the size of the beam waist. This approach creates an exceptionally thin light sheet across a large field of view resulting in images with improved optical sectioning and signal-to-noise. This scanned sheet features a constant laser intensity across the field of view for an evenly illuminated image. The 0.14NA excitation objective creates a 2µm thin waist for superior axial resolution. AxL CTLS is designed to operate optimally across a range of 1.33 to 1.58 refractive indices ensuring compatibility across all available clearing methods.