CapeCod - Model Chromo-LAL -Limulus Amebocyete Lysate

Sensitivity

The sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Chromo-LAL is 0.005 EU/mL.

Sample to BET

Ratio Reconstituted Chromo-LAL reagent is used at a ratio of 1:1 and a volume of 100 μL/well (30 tests/vial)..

Performing the Test

The Chromo-LAL/sample mixture is incubated at 37±1°C and read in a microplate reader. Software is used to construct the standard curve and calculate the endotoxin concentrations.

Reconstitution

Chromo-LAL lysate is reconstituted with BET Reagent Water (LRW). It can also be reconstituted with Glucashield® buffer, a (1g3)-b-D-Glucan inhibiting buffer, to render the assay endotoxin specific.

Stability

Once reconstituted, Chromo-LAL is stable for 24 hours if stored at 2–8°C. Chromo-LAL may be frozen once and will retain activity for 2 weeks if stored at or below -20°C.

Packaging

Each vial contains reagent for approximately 30 tests. It is recommended for use with 0.5 μg/vial Control Standard Endotoxin (CSE, E0005-1). Certificates of Analysis, specific to the Chromo-LAL and CSE lot, can be obtained from ACC or online at www.acciusa.com.