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Repligen LONG - Model R³ IGF-I -Cell Culture Supplement
200 times more potent and two times more stable in cell culture than insulin, LONG® R3 IGF-I (LONG R3) is a recombinant analog of human insulin-like growth factor-I (IGF-I) that has been specifically engineered for the enhancement of cell culture performance.
LONG technology consists of a fusion of the growth factor with a portion of growth hormone sequence. The fusion improves growth factor production without imparting its biological activity.
A distinct biological advantage
All mammalian cells secrete IGF Binding Proteins (IGFBPs) which bind to and inhibit native IGF-I. The substitution of an arginine for glutamic acid at position three in LONG R3 IGF-I, in conjunction with the 13 amino acid N-terminal extension peptide, results in > 1000 fold reduced affinity for IGFBPs, enhancing its bio-availability and effectiveness in comparison to native IGF-I.
Specificity is the key to increased productivity
Unlike insulin, LONG R3 IGF-I specifically targets and activates the type I IGF Receptor (IGF-IR) resulting in greater activation of downstream signaling cascades responsible for proliferation and inhibiting apoptosis. Greater activation of these key pathways results in increased cell density, higher viability and extended cell culture productivity.
To learn more about the use of LONG® R3 IGF-I in stem cell and cell therapy applications, please contact Repligen.
Optimizing Cell Culture
Productivity
Cell culture supplements are essential for long-term growth and productivity of cell lines in serum-free media formulations. Both insulin and LONG® R3 IGF-I, a more potent supplement that directly targets and activates the IGF-I receptor, are employed by the biopharmaceutical industry today to support the growth of recombinant cell lines. This webinar will show how these two supplements, used separately and in combination, impact viable cell density and IgG production of multiple CHO cell lines. It will demonstrate how a combination of LONG® R3 IGF-I and insulin results in less protein supplementation and, therefore, less potential impact on downstream processing.
LONG® R3 IGF-I is a critically important mitogenic growth factor often used in mammalian cell culture to increase yield significantly. Such growth factors are fundamental supplements to chemically-defined media.
LONG® R3 IGF-I is compatible with multiple cell types. Any mammalian cell type that responds to insulin in cell culture has the potential to respond to LONG® R3 IGF-I.
- CHO clones
- HEK 293
- Vero
- MDCK
- Fibroblasts
LONG® R3 IGF-I is available in two formats, both stable at 2-8°C.
LONG® R3 IGF-I can also be milled into cell culture media for additional flexibility and convenience.
Since LONG® R3 IGF-I ships at ambient conditions and stored at 2-8°C, cold chain shipping and storage costs can be significantly reduced.
Freeze-dried powder
- 5 mg (sample size*)
- 50 mg
Ready to use liquid
- 5 mL (sample size*)
- 100 mL
- 250 mL
- 500 mL
- 1L (1000 mL)