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MercuriusModel BRB-seq -Cell Lysate Extraction-Free Library Preparation Kits for Illumina

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RNA-seq library preps directly from cell lysates without prior RNA isolation.

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Why choose our MERCURIUS™ Cell Lysate BRB-seq library preparation kits for Illumina®
The?Extraction-free Cell Lysate BRB-seq kits?contain all the oligos and enzymes needed to go from 2D cell cultures and organoids to sequencing-ready DNA libraries.
Ideal for screening projects

More samples, more?replicates. Robust results, significant discoveries.

Streamlined data pre-processing

Demultiplex and align your BRB-seq data with our easy-to-use cloud-based platform.

No need for prior RNA extraction

An optimized lysis buffer for complete lysis and efficient reverse transcription.

One-day lab workflow
Convenient and short protocol from samples to sequencing-ready libraries in one day.

The generated 3` mRNA-seq data is ideally suited for differential gene expression analysis in the screening setting.

As compared to other bulk 3` mRNA-seq solutions, BRB-seq makes it possible to process virtually any number of cell line samples in one single tube, which helps drastically reduce technical variation, turnaround times and costs.?

The particular aspect of the Cell Lysate BRB-seq workflow is the addition of proprietary lysis buffer, which enables complete lysis of the cellular material while still being compatible with downstream enzymatic reactions.

Each kit contains enough reagents (including Unique Dual Indexing oligos) to prepare up-to four sequencing libraries.

After sequencing, the generated data can be easily demultiplexed and aligned to the genome of choice using our dedicated cloud-based pipeline, which does not require prior bioinformatic experience.

  • For use with (application) 3` mRNA sequencing
  • For use with (equipment) Illumina NGS instruments
  • Validated species Human and mouse cell lines. Organoids.
  • Species compatibility All eukaryotic species
  • Available formats 24, 96, 384, 1536 reactions
  • Shipping conditions Dry ice
  • Storage conditions -20C