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Census - Chlorinated Phenols Remediation
Under anaerobic conditions, pentachlorophenol (PCP) can serve as an electron acceptor for some Desulfitobacterium and Dehalococcoides species. In each case however, complete reductive dechlorination of PCP to phenol was not observed. Instead, PCP dechlorination by Dehalococcoides resulted in the production of a mixture of dichloro- and monochlorophenols. Likewise, Desulfitobacterium strain PCP-1 dechlorinates PCP to 3-chlorophenol but other Desulfitobacterium species are only capable of ortho-dechlorination. Thus the net production of lesser chlorinated phenol must be considered when evaluating reductive dechlorination as a mechanism for PCP biodegradation. QuantArray®-Chlor includes quantification of all Targets listed in the table below. Alternatively, CENSUS® qPCR can be performed to quantify a select subset such as Dehalococcoides and Desulfitobacterium.
| Target | MI Code | Relevance / Data Interpretation |
| Pentachlorophenol Monooxygenases | qPCP | CENSUS assay specifically targeting oxygenase genes encoding the enzymes responsible for initial oxidation of PCP and aromatic ring cleavage. |
| Target |
MI Code |
Relevance / Data Interpretation |
| Desulfitobacterium | DSB | Similar to Dehalococcoides, some species and strains of Desulfitobacterium are capable of utilizing PCP and other chlorinated phenols. Desulfitobacterium hafniense PCP-1 is capable of reductive dechlorination of PCP to 3-CP (Bourchard et al. 1996). However the ability to biodegrade PCP is not universal amoung Desulfitobacterium isolates. Desulfitobacterium sp. Strain PCE1 and D. chlororespirans strain Co23 for example can utilize TCP and DCP isomers but not PCP for growth (Gerritse et al.1996; Sandford et al. 1996). |
| Dehalococcoides | DHC | While the range of compounds utilized varies by strain, some Dehalococcoides isolates are capable of reductive dechlorination of PCP and other chlorinated phenols. For example, Dehalococcoides strain CBDB1 is capable of utilizing PCP and all three tetrachlorophenol (TeCP) congeners, all six trichlorophenol (TCP) congeners, and 2,3-dichlorophenol (2,3-DCP). PCP dechlorination by strain CBDB1 produces a mixture of 3,5-DCP, 3,4-DCP, 2,4-DCP, 3-CP and 4-CP (Adrian et al. 2007). In the same study however, Dehalococcoides mccartyi strain 195 dechlorinated a more narrow spectrum of chlorophenols which included 2,3-DCP, 2,3,4-TCP and 2,3,6-TCP but no other TCPs or PCP. |
