Census - TPH-Alkanes and Crude Oil Biodegradation
From CENSUS (qPCR) – Environmental Remediation
Crude oil and petroleum products are mixtures of hundreds of the different compounds. In addition to BTEX and PAHs discussed under biodegradation of gasoline and diesel, alkanes are important component of petroleum products and contribute to TPH. Under aerobic conditions alkane biodegradation is initiated by monooxygenases. Anaerobic biodegradation of alkanes is initiated by alkyl succinate synthase. Similar to benzylsuccinate synthase which catalyzes the first step in anaerobic biodegradation of toluene, alkyl succinate synthase mediates the addition of fumarate to initiate anaerobic biodegradation of alkanes.
Target
| Target | MI Code | Relevance / Data Interpretation |
| Alkane Monooxygenase | alkB | Catalyzes the aerobic biodegradation of long chain petroleum hydrocarbons. |
| Alkane Monooxygenase 2 | almA | Catalyzes the aerobic biodegradation of C20-C32 alkanes by some Alcanivorax species considered dominant in marine systems. |
| Carbazole Dioxygenase | CAR | Catalyzes the oxidation of carbazole and other high molecular weight aromatics such as dibenzofuran. |
CENSUS Targets for Anaerobic Total Petroleum Hydrocarbons-Alkanes and Crude Oils
| Target | MI Code | Relevance / Data Interpretation |
| Alkyl succinate synthase | assA | Initiates anaerobic biodegradation of alkanes with chain lengths as high as C18. |
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