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cfDNA Extraction Specificity Controls
The Plasma Genomic cfDNA Extraction Control is for the purpose of monitoring the degree of genomic DNA (gDNA) contamination in the extracted cfDNA. DNA-free plasma is spiked with either with high molecular weight gDNA molecules or 170 bp cfDNA fragments or both.
The extraction of cfDNA from patient plasma is a complicated process that causes significant variability from run to run, kit to kit, and operator to operator. Contaminated large-sized genomic DNA interferes with the mutation quantification of cfDNA, which is mainly ~170 bp in size.
Some extraction kits are better in preferentially extracting the 170bp. Figure 4 shows the cfDNA extraction Kit #1 is much better in eliminating the gDNA contamination while maintaining superior extraction yield in 170 bp DNA fragments.
- Screens high molecular weight and DNA fragment loss
- Four types of specificity controls:
- Positive Control Plasma 1 – High MW gDNA +170bp fragments
- Positive Control Plasma 2 – High MW gDNA only
- Positive Control Plasma 3 – 170bp fragments only
- Negative Control Plasma
- Price upon request
