Hichrom - Chiral Columns
Hichrom manufactures two ranges of ‘Pirkle-type’ columns for the analysis of a wide range of enantiomers of pharmaceutical or agrochemical origin. CHIRA-chrom 1 type columns contain a stationary phase in which the hydroxyl groups on the silica surface have been modified by a nitrobenzoyl derivative of an optically active amino acid. In the CHIRA-chrom 2 type columns a chiral dinitrophenyltartramide moiety is bonded to the silica surface through a propyl spacer group.
- Chiral ‘Pirkle-type’
- Unique covalent bonding
- Enantiomer elution order inversion
- Competitively priced
- Hichrom high efficiency
Inversion of enantiomer elution order
By substituting L-phenylglycine for D-phenylglycine, the order of elution of the chiral peaks can be reversed. Such a procedure can be useful in assigning peak identity or ensuring prior elution of the minor enantiomer, thus allowing its more accurate determination. The availability of the DL-phenylglycine further aids peak assignment by removing chiral separations whilst maintaining background peak retention profile.
