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Clean Genome - Model Delta recA -E. Coli Strains
Clean Genome® ΔrecA® is an enhanced strain that is recombination deficient, suitable for production of nucleic acids with structures that may be unstable in recA+ E. coli, such as direct repeats, inverted repeats, stem-loops, etc.
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Quality Control
Transformation efficiency is tested using pUC19 control DNA, performed in duplicate. Transformed cells are plated on LB agar containing 50 μg/mL carbenicillin. Transformation efficiency is ≥ 1 x 108 cfu/μg DNA.
Storage Conditions
Store components at -80 °C. Do not store cells in liquid nitrogen.
