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ALA - CPM-2 (Coating and Polishing Microforge)
Increase efficiency and lower the cost of patch pipette production by using ALA’s CPM-2 Coating and Polishing Microforge. The CPM-2 is designed to be a complete system for processing pulled patch pipettes. It is available as a kit that mounts on a microscope or as a complete system with an inverted microscope.
Increase efficiency and lower the cost of patch pipette production by using ALA’s CPM-2 Coating and Polishing Microforge. The CPM-2 is designed to be a complete system for processing pulled patch pipettes. It is available as a kit that mounts on a microscope or as a complete system with an inverted microscope.
Key Features of the CPM-2 include:
- Convenient control of heat timing with foot-pedal switch
- Heating and air pressure controls conveniently located in one unit
- Coating and polishing without electrode removal
- Available as complete system that includes inverted microscope with parfocal optics or as a kit
Electrophysiologists polish patch pipettes because pipette pulling often leaves sharp surfaces that can damage delicate cell membranes. Coating of pipettes is often necessary with the excised patches or cell-attached configurations to reduce noise from pipette capacitance and dielectric loss1. Pressure polishing, a innovation in pipette processing, minimizes series resistance with small-tip pipettes to facilitate whole-cell recording of small cells2.
1 Hamill, O.P. et al. Improved patch clamp techniques for high-resolution recording from cells and cell free membrane patches. Pflugers Arch. 391, 85-100 (1981).
2 Goodman, M.B. & Lockery, S.R. Pressure polishing: a method for re-shaping patch pipettes during fire polishing. J. Neurosci. Methods. 100, 13-15 (2000).
Each CPM-2 includes the following:
• Air jet with heating element for curing silicone (CPM-HOTJET)
• Polishing filament (CPM-PTIR) with its own XYZ manipulator
• Control box with footswitch
The CPM-2w/scope includes everything in the CPM-2 kit as well as the KXD900 inverted microscope and XY stage manipulator (CPM-XY) for the pipette holder.
The pressure polishing option requires the PR-60, which allows for easy regulation of house or tank pressure, the CPM-PHold bracket and the IPH high pressure pipette holder.
Power Output : 27VDC / 2.5A
Controller Dimensions: 7.65″ W x 9.85″ L x 3.85″ D
Polishing Filament Material: Platinum / Iridium (90%/10%)
Polishing Filament Size: 0.25mm diameter
Polishing Filament Resistance: 4 Ohms
Minimum Input Pressure: 12 psi / 83 kPa
Maximum Input Pressure: 30 psi / 200 kPa
Power Input: 110-120/220-240VAC ~.5/.25A, 60/50Hz
Weight: 6 lbs. 0.2 oz.
| Part # | Description |
| CPM-2w/scope | Coater/Polisher Microforge includes controller, coating & polishing filament, footswitch, XYZ polishing manipulator, pipette holder manipulator, and inverted microscope. |
| KXD900 | CPM Microscope, without stage |
| PR-60 | 60 psi High Pressure Regulator for use in the Pressure Polishing Method |
| CPM-HOTJET | Replacement CPM-2 Hot Air Jet |
| CPM-Polishing | CPM polishing manipulator with Z axis polisihing filament control |
| CPM-PPHOLD | Bracket to hold injection holder for CPM-2 microscope for pressure polishing option |
| CPM-PTIR | PtIr polishing filament – package of 3 |
| CPMXY | CPM-2 XY stage manipulator for pipette movement with pipette clip |
| CPM-Stage | CPM stage assembly for use on KXD-900 microscope, includes CPM XY, CPM polishing and Hot Air Jet |
| IPH-THP | Pressure electrode holder for use with pressure polishing technique |
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