Abbr: Penicillin Amidase, Native (E. coli)
Source: Escherichia coli
Applications: Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N-acylhomoserine lactones) in degradation of antibiotics. It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp. Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.
Product Overview: Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.
Form: Type II, ammonium sulfate suspension, Suspension in 0.1 M phosphate, pH 7.5 and 3 M ammonium sulfate.
Enzyme Commission Number: EC 3.5.1.11
Activity: Type I, 5-10 units/mg protein; Type II, > 10 units/mg protein (E1%/280).
CAS_No: 9014-06-6
Molecular Weight: Mr ~70 kDa
Unit Definition: 1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol benzylpenicillin per minute at pH 7.6 and 37°C
Storage: 2-8°C
Warnings: Characterization; In enantioselective resolution; Synthesis of ampicillin and benzylpenicillin
Synonyms: penicillin amidase; penicillin acylase; benzylpenicillin acylase; novozym 217; semacylase; α-acylamino-β-lactam acylhydrolase; ampicillin acylase; EC 3.5.1.11; 9014-06-6
Publication: Optimization of aqueous two-phase systems for the production of 6-aminopenicillanic acid in integrated microfluidic reactors-separators

Creative - Model NATE-0541 -Native Escherichia Coli Penicillin Amidase

Product Details