CRISPR Associated Transposases (CASTs)

Large gene integrations; Direct­ed DNA inte­gra­tion has large­ly been con­sid­ered the ulti­mate goal of cor­rec­tive genome edit­ing. This tech­nol­o­gy has the poten­tial to address a large col­lec­tion of com­plex genet­ic dis­eases caused by any loss of func­tion muta­tions, such as cys­tic fibro­sis, where inser­tions of greater than 10,000 bp would be required to address all muta­tions. Our CASTs are being devel­oped to per­form these com­plex gene integrations.