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Cytek Aurora - 64 Fluorescence Channels System
A prodigy incorporating a unique combination of patent-pending innovative technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory’s needs, from simple to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a uniquely designed fluidics system, translate to outstanding performance at high sample flow rates.
The end result is a system that delivers high quality data where rare and dim populations are easily resolved, regardless of assay complexity.
SpectroFlo® Software offers an intuitive workflow from QC to data analysis with technology-enabling tools that simplify running applications.
The Cytek team has reimagined every aspect of cytometry hardware and software to deliver an instrument that fulfills every scientist`s needs.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
64 fluorescence channels of detection over the full emission spectra.
Beyond 30 colors demonstrated including fluorochromes with emission spectra in close proximity to each other.
Sensitivity redefined using state-of-the-art optics and low-noise electronics.
No changing optical filters for any fluorochrome.
Use any commercially available fluorochrome excited by the onboard lasers.
A powerful, high value system that is accessible to a wide range of users.
Aurora contains a fixed optical assembly with the capacity to be configured with up to five spatially separated laser beams. Laser delays are automatically adjusted during instrument QC.
LasersBase model three-laser configuration: 405 nm: 100 mW, 488 nm: 50 mW, 640 nm: 80 mW
Available laser upgrades: 355 nm: 20 mW, 561 nm: 50 mW
Flat-Top laser beam profile with narrow vertical beam height optimized for small particle detection.
Emission OpticsEmission CollectionFused silica cuvette coupled to high NA lens for optimum collection efficiency to optical fibers.
Forward and Side Scatter DetectionFSC: high-performance semiconductor detector with 488nm bandpass filter
SSC: two high-performance semiconductor detectors with 405nm and 488nm bandpass filters
Fluorescence DetectorsProprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor array per laser enabling more efficient spectrum capture in the 365-829 nm range. No filter changes required for any fluorochrome excited by the 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers.
Standard Optical ConfigurationViolet detector module: 16 channels unevenly spaced bandwidth from 420-829 nm.
Blue detector module: 14 channels unevenly spaced bandwidth from 498-829 nm.
Red detector module: 8 channels unevenly spaced bandwidth from 652-829 nm.
4 and 5 Laser Options:
Yellow-Green detector module: 10 channels unevenly spaced bandwidth from 567-829 nm.
Ultraviolet detector module: 16 channels unevenly spaced bandwidth from 365-829 nm.
Low: 15 µL/min, Medium: 30 µL/min, High: 60 µL/min, Plate high-throughput mode: 100 µL/min
Fluidic ModesLong clean, SIT flush, Purge filter, Clean flow cell
Manual Sample Input Formats12x75mm polystyrene and polypropylene tubes
Standard Fluidic Reservoirs4L fluid container set with level-sensing provided. Compatible with 20L sheath and waste cubitainers.
Volumetric measurement during sample recording enables calculation of counts per µL for any gated population.
Plate Loader Option96-well microtiter plate capability
Throughput time 35 minutes at High Throughput mode sampling 7 µL/well
Plate stage temperature: 4-30°C
Plate Loader CarryoverDefault mode: ≤0.3%, Low Carryover mode: ≤0.1%, High Throughput mode: ≤1%
PerformanceFluorescence SensitivityFITC: ≤35 MEFL, PE: ≤10 MEFL, APC: ≤10 MEFL, Pacific Blue: ≤25MEFL
* measurements based on an average from two systems, one three lasers and one five lasers, and performed using SPHERO Rainbow Calibration Particle (RCP-30-5A) based on its peak emission channel.
Fluorescence LinearityFITC R2 ≥0.995 / PE R2 ≥0.995
Forward and Side Scatter ResolutionPerformance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Side Scatter ResolutionCapable of resolving 0.2 µm beads from noise.
Carryover≤0.1%
Data Acquisition Rate35,000 events/s*
* Three-laser system
Live unmixing during acquisition
Developed specifically to streamline assay setup, data acquisition, and file export.
Automated QC module
Autofluorescence extraction
Raw and Unmixed FCS 3.1 files
ElectronicsSignal ProcessingDigital signal processing with automatic window gate adjustment.
22-bit 6.5 log decades.
Threshold using any single parameter or combination of parameters.
Pulse Shape ParametersPulse Area and Height for every parameter. Width for scatter parameters and one fluorescence parameter for each laser.
Workstation specifications may vary between laser configuration; below is for three-laser configuration.
Operating SystemWindows® 10 Pro 64-bit
ProcessorIntel® Core™ i7 processor, 3.6 GHz
RAM16GB
Hard Drive500GB SSD / 1TB SATA
Video ProcessorNVIDIA® GeForce
Monitor32” UHD 4K Monitor
Dimensions (W x D x H)
Instrument DimensionsWithout loader: 54 x 52 x 52 cm
With loader: 58.4 x 62 x 52 cm
Instrument weight (4 lasers): 80 kg
Loader weight: 13kg
29.1 x 9.25 x 34.4 cm
Recommended Workspace157 x 71 x 132 cm
100-240V, 50/60 Hz, 2A max
Heat Dissipation500W with all solid-state lasers
Temperature15–28°C
Humidity20%-85% relative non-condensing
Air filteringNo excessive dust or smoke
LightingNo special requirements
For Research Use Only. Not for use in diagnostic or therapeutic procedures.