Biotium - D-Luciferin Free Acid

D-Luciferin is a substrate for the enzyme firefly luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase. Luciferase is encoded by the lucgene, which is widely used as a reporter gene in a variety of cells. Because of the intrinsic low background of chemiluminescence technique, detection of the luc gene expression can be made at very low level. In addition, luciferin/luciferase has been used to measure 10^-15 molar quantity of ATP.

• White solid soluble in DMSO and slightly soluble in water
• Store at -20°C and protect from light
• C₁₁H₈N₂O₃S₂
• Mwt: 280
• [2591-17-5]