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DyLightModel 350 -Donkey Anti-Rabbit IgG (H&L) Conjugated

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Donkey anti-Rabbit IgG (H&L), DyLight 350 Conjugated is a secondary antibody conjugated to DyLight 350, which binds to Rabbit IgG (H&L) in immunological assays. DyLight 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase Rabbit IgG (H&L). DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.

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  • Immunogen: Purified Rabbit IgG, whole molecule
  • Host: Donkey
  • Clonality: Polyclonal
  • Purity: Affinity purified donkey IgG
  • Format: Lyophilized
  • Quantity: 1 mg
  • Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
  • Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
  • For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
  • For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Related products:
  • other DyLight anti-Rabbit secondary antibodies
  • Additional information:
  • Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
  • Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulins
  • No reactivity is observed to: non-immunoglobulin rabbit serum proteins

APPLICATION INFORMATION

  • Recommended dilution: 1 : 20-1 : 2000 for most applications
  • Selected references:
  • to be added when available