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DST - ELISA Kit for Detection of Total IgE
The allergy diagnostics kit quantitatively determines the amount of total IgE antibodies in serum or plasma, for the diagnosis of an existing type 1 allergy.
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- 88 individual tests are possible with every kit
- 12 microtiter plate strips are included, each with 8 wells.
- Also includes 8 wells that are pre-coated with standards and controls
- 10 μl serum or plasma is required
- Incubation period of 60 minutes
- The result provided in concentration range of 0 – 1,000 U/ml.
The quantification of total IgE is based on a sandwich ELISA protocol (Enzyme Linked Immunosorbent Assay). The wells of the microtiter strips are coated with polyclonal anti-IgE antibodies which bind the existing immunoglobulin from the sample. Detection occurs with a HRP conjugate (horseradish), which makes the visual evaluation and quantification of bound antibodies possible. The results are analysed photometrically at 405 nm after the reaction is stopped.
| Age in years |
U/ml |
| Newborns | < 2,0 |
| < 1 | 0 - 10,0 |
| 1 - 4 | 0 - 47,5 |
| 4 - 16 | 0 - 150,0 |
| > 16 | 0 - 120,0 |