BioGenes - Model CHO|360-HCP -ELISA Kits

To show this, we tested CHO|360-HCP ELISA kits (A to D) and a commercially available generic CHO HCP assay, on the basis of a great number of mock CHO HCP samples. In most cases, the best recovery was achieved with one of the four CHO|360-HCP ELISAs kits, as compared to other commercial kits (see right-hand image). 

• Sample 4: a recovery value of > 90% was estimated with ELISA kit type D while ELISA kits A, B and C and the commercial kit were less sensitive, with the latter detecting only 20% of the host cell proteins.
• Recovery values > 100% may occur due to overestimation.

Why does BioGenes apply HCP fractionation prior to immunization?

Naturally, low molecular weight (LMW) proteins are less immunogenic than larger proteins, leading to a reduced production of anti-LMW HCP antibodies after the immunization of animals. Consequently, very weakly immunogenic or non-immunogenic HCPs may not be detected by ELISA or Western Blot. The BioGenes`

CHO|360-HCP ELISA solved this by using the total and fractionated HCP for the immunization of animals, resulting in four different HCP ELISA kits (A to D).

Antibodies obtained from immunization with each of three different molecular-weight fractions were then pooled again to combine antibody reactivity towards to the complete HCP spectrum. The immunization of rabbits and goats enables the generation of distinct antibody panels, which allows for a broader coverage of HCPs in customized samples.

The BioGenes recommendation:

We recommend testing all four kit types (Starter Set) first, and then selecting the best performing kit according to ELISA key parameters (such as dilution linearity, accuracy and total coverage) for use in all subsequent tests.