Evosep One - Model EV-1000 -Standardized Platform Designed for Robustness and High Throughput
The Evosep One is a standardized platform designed for robustness and high throughput. This is achieved by using Evotips as disposable trap columns, where samples are loaded and desalted offline. The autosampler picks up the tip and integrates elution with liquid chromatography. This removes sample handling steps and reduces injection cycle overheads. Minimal wear and tear on mechanical components is ensured as all elution and gradient formation happen at low pressure. The Evotip is left for disposal after each run, which then allows partial elution to extend column lifetime. Analytically important peptides are effectively retained, and cross-contamination reduced.
The Evosep One provides a standardized solution with excellent reproducibility both “run-to-run”, “column-to-column” and “instrument-to-instrument”, facilitating individual studies and inter-laboratory collaborations alike.
Illustrated below using the 100 samples per day method and the standard column (EV-1064).
With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis.
The speed of state-of-the-art mass spectrometers combined with the low overhead time and robustness on the Evosep One is a perfect match for high utilization. It is therefore needed to carefully review the balance of proteome depth versus high throughput before starting a series of experiments.
Our five standard methods cover a broad range of applications. Explore more and find your favorite.
30 SPD: The longest method with the best overall proteome coverage. Download app note
60 SPD: The most popular method with an excellent balance of throughput and depth. Download app note
100 SPD: A great “in-between” method. Download app note
200 SPD: Excellent sequencing speed with most peptides per minute among our standard methods. Download app note
300 SPD: The highest peak capacity per minute with our fastest method for ultra-high throughput analysis. Download app note