AventaCell Helios - Model UltraGRO™-PURE GI -Exosome-Depleted, Gamma Irradiated FDhPL - Research grade
Exosome-Depleted UltraGRO™-PURE GI cell culture supplement is a gamma irradiated viral inactivated, exosome-depleted, xeno-free to support cell culture, especially for exosome secretion and isolation assay from research and clinical trials to commercial applications. More than 95% of nanoparticles have been removed from the original human platelet lysate, allowing target cells to produce functional exosomes and other cell-derived extracellular vesicles for further purification, analysis, and therapeutic purposes.
- Endotoxin: ≤ 10 EU/mL
- Sterility: No Growth
- Mycoplasma: Negative
- Osmolality: 270 - 330 mOsm/Kg
- pH: 6.5 - 8.5
- Particle depleted rate: >95%
- Cell assay: Support MSC culture
- Gamma Irradiation Dose: 25~40 kGy
Exosome-Depleted UltraGRO™-PURE GI is most stable when stored frozen until needed. The recommended storage temperature is -20°C. Thaw frozen Exosome-Depleted UltraGRO™-PURE GI product in 37°C water bath before use. Once Exosome-Depleted UltraGRO™-PURE GI is thawed, it is recommended to fully use for completed medium preparation (e.g., 5%) on the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C. The product will be shipped with dry ice.
Application:
For human cell culture in producing exosome and other cell-derived extracellular vesicles processing applications.
Instructions for Use:
- Once the target cells reach desired cell confluency, Exosome-Depleted UltraGRO™-PURE GI supplemented culture media can be introduced to initiate cell-derived EV production.
- We recommend applying with 2-5% (v/v) of supplemented media during the culture period. Perform conditioned media harvest and media refresh in every 2-3 days.
Cell Lines:
bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells
Important Information:
- Insoluble particles may form in thawed Exosome-Depleted UltraGRO™- PURE GI. Published research has shown that particles will not alter the performance of the product. It is recommended to remove the precipitates by centrifuge at 3,400 xg for 3~5 minutes. Alternatively, applying 0.22 µm filtration to the prepared Exosome-Depleted UltraGRO™-PURE GI containing medium (e.g., 5%) will not affect the cell culture performance.
- 0.22μm filtering is NOT recommended for the 100% Exosome-Depleted UltraGRO™-PURE GI.
- Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates.