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Jena Bioscience GmbH
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  5. Fast qPCR ProbesMaster - Model PCR-383 ...

Fast qPCR ProbesMaster - Model PCR-383 -Fast Master Mix Kit

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Fast master mix for quantitative real-time PCR using labeled DNA probes. Fast qPCR ProbesMaster is designed to perform rapid real-time analysis of DNA samples using DNA probe based detection. The master mix is recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan®, Molecular Beacons or FRET probes. The mix contains all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a premixed 2x concentrated ready-to-use solution.

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For in vitro use only!

  • Shipping:shipped on blue ice
  • Storage Conditions:store at -20 °C avoid freeze/thaw cycles  Storage at 4 °C for up to 3 months possible.
  • Shelf Life:12 months
  • Form:liquid
  • Concentration:2x conc.

Description:

Amplification within 30-60 min combined with high specificity and sensitivity is achieved with an optimized hot-start polymerase. Its activity is blocked by an antibody at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.

The mix can also be used in combination with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal.

Dual-labeled DNA probes:
Real-time PCR technology based on dual-labeled DNA probes provides a high sensitive and specific PCR system with multiplexing capability. It requires two standard PCR primers and the DNA probe that hybridizes to an internal part of the amplicon. The sequence of the dual-labeled DNA probe should avoid secondary structure and primer-dimer formation.

Content:
Fast qPCR ProbesMaster (red cap)
antibody-blocked fast hot start polymerase, dATP, dCTP, dGTP, dTTP, KCl, (NH4)2SO4, MgCl2, additives and stabilizers

PCR-grade water


Preparation of the qPCR master mix:
The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Prepare a master mix of all components except template as specified. A reaction volume of 20-50 μl is recommended for most real-time instruments. Pipet with sterile filter tips and minimize the exposure of the labeled DNA probe to light. Perform the setup in an area separate from DNA preparation or analysis. No-template controls should be included in all amplifications.