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Biosense Laboratories AS - Model V01018401 -Fathead Minnow Vitellogenin ELISA Kit
The quantitative Fathead minnow (Pimephales promelas) Vitellogenin (Vtg) ELISA Kit is developed for a standard OECD test species used in many ecotoxicological testing laboratories throughout the world, the fathead minnow (Pimephales promelas). The assay can be used for analysis of both blood plasma and whole body homogenate samples). Detection of the egg yolk protein vitellogenin (Vtg) in juvenile and male fish is a simple and sensitive biomarker for endocrine disrupting chemicals (EDCs) with estrogenic effects in fish. Measuring Vtg has become accepted as a routine screening test for estrogenic effects of EDCs in fish. The Fathead minnow Vtg ELISA Kit comes in two sizes, with 96 and 480 wells (1 and 5 plates). The kit contains pre-coated microtiter plates, fathead minnow Vtg standard, antibodies and reagents necessary for running the assay, as well as an instructive protocol.B. SAFETY INSTRUCTIONS
For research use only. Not for human use or drug use. Not for clinical diagnostic use. These reagents contain sodium azide as preservative. Do not use internally or externally in humans or animals. As all chemicals should be considered potentially hazardous, it is advisable to wear suitable protective clothing during handling of this kit. Avoid contact with skin and eyes.
C. STORAGE AND STABILITY
Store the kit at 2-8°C upon arrival. Do not freeze. See expiry date on the kit box for stability of the kit. Unused pre-coated microplates should be stored airtight with enclosed desiccant at 2-8°C.
D. WARRANTY AND LIMITATION OF REMEDY
Biosense Laboratories AS (hereafter: Biosense) makes no warranty of any kind, expressed or implied, including, but not limited to, the warranties of fitness for a particular purpose and merchantability, which extends beyond the description of the chemicals on the face hereof, except that the material will meet our specifications at the time of delivery.
Buyer’s exclusive remedy and Biosense´s sole liability hereunder shall be limited to refund of the purchase price of, or at Biosense´s option, the replacement of, all material that does not meet our specifications. Biosense shall not be liable otherwise or for incidental or consequential damages, including, but not limited to, the costs of handling.
Said refund or replacement is conditioned on Buyer giving written notice to Biosense within thirty (30) days after arrival of the material at its destination, and Buyer treating the material as outlined in the product data sheet and/or kit insert after arrival. Failure of Buyer to give said notice within said thirty (30) days, or failure of Buyer in treating the material as outlined in the product data sheet and/or kit insert shall constitute a waiver by Buyer of all claims hereunder with respect to said material. The responsibility of all patent considerations in the use of our products rests solely with the user.
E. ASSAY PRINCIPLE
This ELISA utilises specific binding between antibodies and vitellogenin (Vtg) to quantify Vtg in samples from fathead minnow (Figure 2). The wells of microplates have been pre-coated with a specific Capture antibody that binds to Vtg in standard and sample added to the wells. A different Vtg-specific Detecting antibody, labelled with the enzyme horseradish peroxidase (HRP), is added to create a sandwich of Vtg and antibody. The enzyme activity is determined by adding a substrate that gives a coloured product, and the colour intensity is directly proportional to the amount of Vtg present.