Fivephoton Biochemicals
FIVEphoton - ELISA Kits
Illustrations of kit formats offerred by Fivephoton Biochemicals.
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Format I
ELISA Assay Steps
- Mix sample, biotinylated-detection antibody and streptavidin-HRP.
- Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC. Wash 5 times.
- Apply chromogens, incubate, then stop reaction.
Format II
- Equilibrate plate to RT
- Add sample to plate, incubate overnight
- Add detection antibody, incubate 1 hr
- Add color reagents, 30 min
- Add stop solution. Measure OD at 450nm
Format III
ELISA Assay Steps
- Mix sample, biotinylated-detection antibody and streptavidin-HRP.
- Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.
- Apply chromogen incubate, then stop reaction.
