Fivephoton Biochemicals

FIVEphotonELISA Kits

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Illustrations of kit formats offerred by Fivephoton Biochemicals.

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Format I
ELISA Assay Steps
  1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.
Format II
  • Equilibrate plate to RT
  • Add sample to plate, incubate overnight
  • Add detection antibody, incubate 1 hr
  • Add color reagents, 30 min
  • Add stop solution.  Measure OD at 450nm

Format III
ELISA Assay Steps

  • Mix sample, biotinylated-detection antibody and streptavidin-HRP.
  • Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.
  • Apply chromogen incubate, then stop reaction.