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FIVEphotonModel APC-1-FIVE -Allophycocyanin (APC) Fluorescent Protein

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Allophycocyanin (APC) is a fluorescent protein with high absorbtivity and high quantum efficiency and can be conjugated to proteins for fluorescent detection.  Allophycocyanin is attached to proteins using standard cross-linking techniques. Proteins conjugated with APC are used for cell sorting, flow cytometry, FRET, and ELISA.  APC is isolated from cyanobacteria algae in the Spirulina genus.

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  • Source:  Cyanophyta`
  • Subunit Stucture: (αβ)3
  • Molecular Weight:  105kDa
  • Absorption Maximum:  651 ± 2 nm
  • Emission Maximum:  660 ± 2 nm
  • Extinction Coefficient: 7.0 x 105 cm-1M-1
  • Protein Concentration: ? 15g/mL
  • Purity:  A651/A280 ≥ 5.0; A651/A620 ≥ 1.5
  • Stability:  Supplied in 100 ml sodium phosphate buffer pH7.0 with 60% ammonium sulfate, 1mM EDTA, and 1mM sodium azide
  • Shelf Life: 2 years with proper storage
  • Storage: Store at in the dark at 4°C.  Do not freeze.  Composition:  Liquid