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Microbiotests - Model Algaltoxkit F -Freshwater Algae Toxicity Test
The freshwater algae toxicity test is intended for toxicity screening of chemicals, effluents, surface waters, wastewaters, groundwaters, sediment pore waters and elutriates. Algaltoxkit F contains all the materials you need to perform two 72h growth inhibition tests with the freshwater microalgae Pseudokirchneriella subcapitata (former names: Selenastrum capricornutum and Raphidocelis subcapitata). This cost-effective and culture-independent Algal toxicity test strictly adheres to ISO Standard 8692 and OECD Guideline 201.
The freshwater algae toxicity test is a 72h bioassay based on growth inhibition of the freshwater microalgae Pseudokirchneriella subcapitata, with calculation of the 72h EC50.
- Tubes with algal beads which you can de-immobilise on demand in less than 30 min to supply the live biota for the freshwater algae toxicity test.
- Concentrated algal growth and toxicant dilution medium, long cell cuvettes and holding trays.
- Short Bench Protocol and standard operating procedure brochure with easy-to-follow instructions and detailed illustrations.
- OD/N regression sheet, data scoring sheets and a Quality Control specification sheet with batch number of the algal beads and the media.
All test materials and equipment included in the Algaltoxkit F are available separately.
- We produce high-quality algal beads in strictly controlled conditions. This way, we preclude variability associated with recruitment/maintenance of live stocks of microalgae in exponential growth phase in conventional bioassays.
- The quality of the test medium is highly uniform, thanks to simple dilution of concentrated solutions of selected chemicals with deionised water.
- A Quality Control test with a reference chemical is described in detail, so you can easily check accuracy and reproducibility.
- The Algaltoxkit F uses unique disposable “long cell” exposure chambers (10 cm path length), which serve as measuring cuvettes for direct determination of algal growth by optical density measurements.
- If you store the ephippia properly, you can prolong their shelf life for several months and reduce test scheduling constraints.
- The freshwater algae toxicity test requires minimal space and equipment:
- tabletop centrifuge
- spectrophotometer
- incubator with lateral lights
- conventional laboratory glassware
- If you store the algal beads properly, you can prolong their shelf life for several months and reduce test scheduling constraints.
- Simple handlings and scorings.
- You can start the freshwater algae toxicity test within 30 min after you’ve de-immobilised the microalgae from the algal beads.
- Daily scoring of the optical density of the microalgae in the long cells takes less than 15 min.
- We can provide you with a computer program for easy Toxkit data treatment.
The sensitivity of the Algaltoxkit F toxicity test is comparable to the sensitivity of the conventional algal growth inhibition test with microalgae from laboratory cultures.
- The freshwater Algaltoxkit F is used in many environmental laboratories and research institutes worldwide and is validated by extensive international interlaboratory comparisons.
- You can find a substantial number of publications, posters, reports and reviews for various uses.
- Our freshwater algae toxicity test complies with the protocols ISO Standard 8692 and OECD Guideline 201, for regulatory testing with microalgae.