Giemsa Stain

Reagent 1
Methyl alcohol, absolute (analar)

Reagent 2
Giemsa Stain

1. Prepare thin blood films and fix in Reagent 1 for 3 min
2. Dilute one volume of Reagent 2 with four volumes of distilled water, flood the slide and allow the stain to act for 5-20 min.
3. Wash and differentiate with dist.water, controlling the degree of differentiate microscopically.
4. Drain and dry in the air at room temperature.

• Nuclei of leucocytes : Reddish-purple.
• Eosinophilic granules : Red to orange.
• Basophilic granules : Blue.
• Lymphocytes : Dark purple.