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Thomson - Model BIOAdvantage Basic -High Efficiency HPLC & LC Columns
BioAdvantage Basic high efficiency columns give excellent peak shapes without modifiers. Polar molecules are a problem for most silica based columns. Their polar surface interacts with the solute, sometimes proving critical to the separation, but in the case of polar amines, often causing them to tail badly.
BioAdvantage Basic for Polar Molecules
This can be overcome with ionic modifiers such as buffers and TFA which either “tie” up the solute to prevent this interaction or shield the residual silanols from the solute.
BioAdvantage Basic eliminates the need for these modifiers. It’s illustrated by the chromatograms below without mobile phase “helpers” compared to very popular columns attempting the same thing.
- Column: BioAdvantage Basic C18, 5µm, 4.6 x 100mm
- Mobile Phase: 40% AcCN/Water, 1ml/min, 254nm.
- No buffers or modifiers
- Peak Identities: 1. Uracil 2. Pyridine 3. Phenol
The chromatograms below illustrate the power of changing modifies to effect changes in elution order. BioAdvantage Basic, C18, 5µm, 1.0 x 50mm. Elution with 0.1% acetic acid versus Elution with 0.1% TFA.
BioAdvantage HL, High Load, is intended for separations requiring maximum loading and selectivity. The high carbon load, 18%, and absence of micro-pores eliminates the problems of other high capacity columns.
- Column: BioAdvantage HL C18, 5µm, 4.6 x 150mm
- Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
- Gradient: Linear gradient, 1 ml/min, 0 to 30% solvent B in 30 minutes.
- Detection: UV 210 nm
- Discussion: The Canadian corporation, SPI, produces a well characterized, challenging peptide mixture that is very useful for quantitying HPLC column performance for peptides. The difficult to separate first pair of decapeptides are near base line resolved on Advantage HL C18 columns.
Peak Identities:
- H2N-RGAGGLGLGK-amide
- AC-RGGGGLGLGK-amide
- AC-RGAGGLGLGK-amide
- AC-RGVGGLGLGK-amide
- AC-RGWGLGLGK-amide
BioAdvantage 100, a work horse, for years has been the choice for separations of small molecules like carbamate pesticides, pharmaceuticals, synthetic oligonucleotides and small peptides. It is characterized by high efficiency symmetrical peaks and long life
- Column: BioAdvantage 100, C18, 5µm, 4.6 x 250mm
- Mobile Phase: A: 50 mM phosphoric acid, pH 2.8
- B: 60% Acetonitrile / 40% A
- Gradient: 1 mi/min
- 0 min 0%B
- 5 min 0%B
- 120 min 40%B
- 130 min 60%B
- Detection: UV 215 nm
- Instrument: PerSeptive Biosystems Integral
- Column: BioAdvantage HL, C18, 5µm, 4.6 x 100mm
- Mobile Phase: A: 70% Acetonitrile in water
- Detection: UV 254 nm
- Discussion: Fast analysis, symmetrical peaks and baseline resolution are characteristics of paraben analysis on short, high carbon load C18 columns.
Peak Identities:
- Methyl paraben
- Ethyl paraben
- Propyl paraben
- Butyl paraben
- Column: BioAdvantage 100, C18, 5µm, 4.6 x 100mm
- Mobile Phase: A: 0.1M TEAA, pH 7.0
- B: Acetronitrile
- Gradient: Linear, 1ml/min from 8-15% ub 24 mins.
- Detection: UV 260 nm
- Discussion: Trityl-off chromatography is a routine method used for purification and purity determination of synthetic oligonucleotides.
BioAdvantage Basic is designed for polar molecules such as amines and pharmaceuticals. Eliminates tailing without the need for mobile phase modifiers. Perfect for LC-MS applications. C18 and C8, 3, 5 & 10µm.
BioAdvantage BHS is a high surface area version of BioAdvantage Basic. 30% more surface for very difficult separations. C18, 5 & 10µm.
BioAdvantage CL has selectivity more similar to other base deactivated phases but, unlike the others, doesn’t need mobile phase modifiers. Perfect for LC-MS applications. C18, C8, Cyano, Phenyl and Silica, 5 & 10µm.
BioAdvantage HL is a high load C18 (18%) designed for difficult separations requiring maximum selectivity. C18, 5µm.
BioAdvantage Pro200 (200Å) is designed for biomolecules, peptides and small proteins. C18 and C4, 5 & 10µm.
BioAdvantage Pro300 (300Å) is designed for biomolecules, peptides and small proteins. Pro300 & Pro200 phases complement each other, extend the upper MW range for peptides and small proteins and exhibit identical selectivities. C18 and C4, 5 & 10µm. These columns are a perfect replacement for Vydac® 218TP and 214TP columns.
BioAdvantage 100 is general purpose RP column on Type A silica. C18, C8 and Silica, 3 & 5µm.
BioAdvantage 300 is designed for biomolecules, peptides and small proteins. C18, 5 & 10µm.
Many different configurations are available. If you don’t find the one for which you’re looking, we hope you’ll ask. Chances are good that we provide it.