HOT FIREPol SolisGreen - Highly Sensitive Dye-Based qPCR Mix
Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye. It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. SolisGreen® dye is characterized by high sensitivity and
great PCR efficiency with low template amounts for accurate and reproducible results
- improved detection of low target concentrations
- higher fluorescence level
- reaction set-up and shipment without dry ice
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
- Concentration: 5x
- Hot-start: yes, initial activation in 10 min
- Detection type: dye-based, includes SolisGreen® intercalating dye
- Reference dye: based on ROX
- Compatible real-time instruments: Most qPCR cyclers except platforms that require high ROX (HOT FIREPol® SolisGreen qPCR Mix is not compatible with the Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems.) Check Your cycler!
- HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
- 5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
- dNTPs: dATP, dCTP, dGTP and dTTP
- SolisGreen® dye
- Internal reference based on ROX dye
Properties
Concentration: 5x
Hot-start: yes, initial activation in 10 min
Detection type: dye-based, includes SolisGreen® intercalating dye
Reference dye: based on ROX
Compatible real-time instruments: Most qPCR cyclers except platforms that require high ROX (HOT FIREPol® SolisGreen qPCR Mix is not compatible with the Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems.) Check Your cycler!
Mix Components
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
dNTPs: dATP, dCTP, dGTP and dTTP
SolisGreen® dye
Internal reference based on ROX dye
SolisGreen® dye
This dye-based qPCR mix contains next-generation DNA binding dye SolisGreen®. The fluorescence spectra of this dye is similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. SolisGreen® dye shows:
- high fluorescence level
- high sensitivity for detecting low template concentrations
- high stability for room temperature storage