Holloid - Real-Time 3D Microscopy System for Online Microbial Monitoring
Holloid offers a pioneering real-time 3D microscopy system tailored for label-free analysis, bridging the gap between high-quality microscopy and high-throughput colloid analysis. Utilizing in-line 3D holography, the technology records holograms to create 3D image data. Coupled with numerical back-propagation algorithms, the system generates bright-field images encompassing phase information. This combination enhances contrast simulation and object characterization, enabling the detection and categorization of every object within a sample volume based on multiple parameters.
Traditionally, you had to choose between high-quality microscopy and high-throughput analysis of colloids.
Holloid delivers both.
In-line 3D holography
Light passes through a sample, and an image is recorded that contains holograms—i.e., 3D image information.
Numerical back-propagation
Algorithms generate a stack of bright-field images that include phase information.
Contrast simulation & novel algorithms
This information is used to simulate different contrast methods, including new and advanced ones.
Object characterization
All objects in a sample volume can be detected and characterized across multiple parameters.
Dynamic tracking of all objects
Objects tracked over time can be dynamically characterized using various metrics.
Our technology creates sharper images with higher contrast and can therefore discriminate particles that others miss. It’s real-time, label-free 3D microscopy, which enables high-throughput analysis and continuously captures millions of objects. Thanks to our precise algorithms, you can get actionable data faster.
Holloid provides real-time multi-dimensional data on physical characteristics. We can quantify the following physical properties in real-time for all objects based on 3D imaging with the throughput of cytometry.
Static
- Number
- Size
- Shape
- Amount of scattered light (dry mass)
- Inner structure (e.g., centrosome, inclusion bodies)
Dynamic
- Motility
- Translational diffusion (hydrodynamic size)
- Rotational diffusion (asymmetric objects)
- Sedimentation rate (density) Interaction potentials