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AmpersandModel CgA mAb (cGA-2G8) M2009 -Human Antibodie

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Chromogranin A (CgA), UniProt # P10645, is a 439 amino acid member of the granin family of secretory proteins. Found in vesicles of neuroendocrine cells, CgA is considered a valuable marker for neuroendocrine tumors. CgA is a precursor to a number of biologically active peptides including vasostatin I, parastatin, and pancreastatin.

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Description

  • Immunogen: E. coli-derived recombinant human CgA. Pro24-Gly457..
  • Reactivity: Human
  • Source: Mouse monoclonal IgG2b
  • Purification: Protein G purified from hybridoma cell culture supernatant
  • Applications: ELISA, IP, WB
  • Formulation: Lyophilized from PBS with Trelahose
  • Reconstitute in 100μL ddH20 to 1 mg/mL
  • Shipping: Ambient
  • Storage
    • 5 years at -20°C to -80°C as supplied
    • 1 month at 4°C after reconstitution with preservative
    • 1 year at -20°C to -80°C after reconstitution
    • Expiration: See lot specific CoA

Human CgA Luminex Assay

  • Capture: Human CgA monoclonal clone CgA-3H11 (Cat # M2007)
  • Detection: Human CgA monoclonal clone CgA-2G8 (Cat # M2009)
  • Antigen: Recombinant human CgA, E. coli expressed (Cat # P4006)

Immunoprecipitation of native human CgA

  • SDS PAGE 4-12%, reducing conditions
  • Blocking: 5% NFDM, PBS
  • Sample: Human CgA purified from human serum with M2009
  • Blot was probed with 4μg/mL M2009 followed by HRP detection antibody. Human CgA was detected at 52kDa.