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NexcelomModel Cellometer Spectrum -Image Cytometry System

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The Spectrum Image Cytometer is a customizable cell counting and cytometry instrument that provides flow-like data with predesigned templates. Quickly plot cell population data as a histogram, scatter plot, dot plot or contour plot using FCS express. The filter sets are user-changable, allowing you to customize the instrument for specific assays/reagents of interest.

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All-in-One System

Basic cell counting, primary cell viability, and cell-based assays.

Dual-Fluorescence for Accurate Primary Cell Viability

No interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.

User Changeable Fluorescence Filters

Choose from six color options to run two-color assays

Unique Algorithms for Advanced Cell Analysis

Determine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.

Fast Results

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

Simple Cell-Based Assays
  • Small 20 µl sample size
  • Cell count, concentration and viability
  • Two-color antibody assays
  • GFP/RFP Transfection
  • Cell health and cell-based assays, including:
    • Apoptosis
    • Cell Proliferation
    • Cell Cycle
    • Mitochondrial Potential
    • Phagocytosis
    • Surface Marker Analysis
Advantages of Image Cytometry

View Cell Images

  • Visually check cell morphology
  • Ensure only cells of interest are counted
  • Archive and re-analyze cell images
  • Export images for publication

Proprietary Pattern-Recognition Software

  • Count individual cells in clusters
  • Count irregular-shaped cells
  • Count cells based on size
  • Eliminate debris from cell counts

Non-Fluidic Platform

  • Disposable counting chambers – no washing
  • Compatible with fragile cells
  • Maintenance-free
  • Robust optics modules and LED light sources

IQ/OQ Validation and GMP/GLP Accessories

  • Installation Qualification reagents/protocol
  • Operational Qualification reagents/protocol
  • On-site IQ or OQ Performance
  • GMP/GLP Software Module
Accurate Cell Counting
  • Automatic identification of Acridine Orange (AO) stained cells (shown above)
  • Image and count up to 2×107 cells/mL
  • Image acquisition and analysis: 30 seconds/sample for a 3-channel assay
  • Versatile platform – use for routine cell counting and complex cell-based assays
Proven Results
  • Determine cell viability, for cell lines or primary samples, using AO/PI in seconds
  • Accurately measure cell samples with varying viability (0 – 100%)
Export to FCS Express* for Flow-Like Data
  • Export image data into flow cytometry software: FCS express
  • Simple workflow: No fluid- stream, No PMT voltages, No forward/side scatter
  • Easily perform data analysis using pre-designed templates
  • Quickly plot cell population data as a: histogram, scatter plot, dot plot or contour plot

User-Changeable Fluorescence Optics Modules*

The Spectrum is designed to hold two user-changeable fluorescence optics modules. Purchase only the modules you need and easily configure the instrument by quickly changing the colors to fit your experimental design.

Optimized for primary cell analysis
PBMCs

Stem Cells

Adipocytes

Neural Cells

Hepatocytes

Dendritic Cells

Epithelial Cells

Keratinocytes

Lymphocytes

Splenocytes

Monocytes

  • Clinical Immunology: PBMCs
  • Diabetes / Obesity: Adipocytes
  • Immunotherapy: Leukocytes
  • Microbiology: Yeast (Spectrum 10x)
  • Oncology: Cell Lines
  • Regenerative Medicine: Stem Cells
  • Toxicology: Hepatocytes
  • Transplantation: Nucleated Cells
  • Vaccine Development: Splenocytes

Cell Counting / Viability Reagents Yeast Viability & Vitality Reagents (for 10x instrument) Apoptosis Reagents Cell Cycle Reagents Proliferation/Tracer Reagents Cell Health/Fitness Reagents

Includes

  • Cellometer Spectrum Instrument
  • Cellometer Software
  • FCS Express 6 analysis software (2 licenses)
  • Two Fluorescent Optics Modules
  • Power Supply
  • USB 2.0 Connection Cable
  • Phone/online applications support during set-up

Magnification Selection

  • 5x (ex: most mammalian cells)
  • 10x (ex: yeast, platelets, algae)

Imaging Performance

5x magnification
Cell Size: 5 – 300* microns, Conc. Range: 105 – 107 cells/ml10x magnification
Cell Size: 0.5 – 25 microns,  Conc. Range: 105 – 107 cells/mlBright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.* Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter

Instrument Specifications

  • Weight: 24.0 lbs. (10.9 kg)
  • Dimensions: Width: 6.0” (15.2 cm), Depth: 8.5” (21.6 cm), Height: 14.0” (35.6 cm)
  • Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A
  • Output to Instrument: 12 VDC, 3.34A

PC / Laptop
Minimum
Requirements(If purchasing Cellometer  without PC laptop)

  • Windows 10
  • 2 GHz or higher processor
  • 4GB RAM (8GB RAM or higher recommended)
  • USB 2.0 or higher
  • Display resolution: 1024 x 768 pixels or higher

Intel Mac with Parallels/Running Windows 10