FlowCytobot - Model IFCB -In-Situ Automated Submersible Imaging Flow Cytometer
The Imaging FlowCytobot (IFCB) is an in-situ automated submersible imaging flow cytometer that generates images of particles in-flow taken from the aquatic environment. The IFCB uses a combination of flow cytometric and video technology to capture high resolution images of suspended particles. Laser- induced fluorescence and light scattering from individual particles are measured and used to trigger targeted image acquisition; the optical and image data are then transmitted to shore in real time.Collected images during continuous monitoring can be processed externally with automated image classification software. Images can be classified to the genus or even species level with demonstrated accuracy comparable to that of human experts.
IFCB generates high resolution (~3.4 pixels/micron) images of suspended particles in the size range less than 10 to 150 μm (such as diatoms and dinoflagellates). The instrument continuously samples at a rate of 15ml of sea water per hour. Depending on the target population the IFCB can generate on the order of 30,000 high resolution images per hour.
- DEPLOYMENT: Typical deployment 6 m (longer possible)
- DEPTH: Maximum depth of 40 m
- TEMPERATURE RATING: Temperature 0 to 45 °C
- IMAGE RATE: 30,000 images per hour (depending on target)
- RESOLUTION: High resolution (~3.4 pixels/micron)
- POWER: Requires 18-36 VDC external power source
- POWER CONSUMPTION: Consumes 35 W (continuous sampling)
- SCHEDULE: Automated or interactive system