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1,2-Dioleoyl-sn-glycero-3-phosphocholine

MCE China：1,2-Dioleoyl-sn-glycero-3-phosphocholine

Brand：MedChemExpress (MCE)

Cat. No.HY-113424A

CAS：4235-95-4

Synonyms：DOPC

Purity：98.11%

Storage：-20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Shipping：Room temperature in continental US; may vary elsewhere.

Description：1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) is a phospholipid and is commonly used alone, or with other components, in the generation of micelles, liposomes, and other types of artificial membranes.

In Vitro：Application of DOPC in biochemical experiments1. Liposome constructionDrug delivery carrier: DOPC is widely used in liposome preparation due to its high membrane stability and low cytotoxicity. For example, liposomes formed by mixing DOPC and cholesterol (2:1 molar ratio) can efficiently encapsulate hydrophobic drugs (such as paclitaxel) with a drug loading efficiency of more than 85%, and achieve tumor-targeted delivery through enhanced permeability retention (EPR)[1].Gene transfection assistance: DOPC is combined with cationic lipids (such as DOTAP) to form lipid complexes, which can reduce the cytotoxicity of cationic lipids and improve the transfection efficiency of plasmid DNA or siRNA[2].2. Membrane protein researchMembrane protein reconstitution: DOPC is a commonly used phospholipid for the construction of nanodiscs. Together with membrane scaffold protein (MSP), it forms a phospholipid bilayer structure with a diameter of about 10 nm, providing a natural membrane environment for membrane proteins (such as GPCRs) to maintain their conformation and function[3].Electrophysiological studies: DOPC is used to form a planar lipid bilayer (BLM), and combined with the patch clamp technique, the conductivity characteristics and gating mechanism of ion channels (such as potassium channels) are studied[4].3. Cell membrane structral studiesMembrane fluidity analysis: DOPC lipid bilayers are labeled with fluorescent probes (such as DPH) to measure their microdomain fluidity and quantify the effect of cholesterol on membrane order (for every 10 mol% increase in cholesterol concentration, membrane fluidity decreases by 15%)[5].Molecular interactions: Using surface plasmon resonance (SPR) technology, the affinity of the antimicrobial peptide LL-37 to DOPC membranes was measured (KD ≈ 1.2×10⁻⁶ M), revealing its membrane damage mechanism[6].4. Cell toxicity assessmentToxicity control: As a neutral phospholipid, DOPC is often used as a negative control to evaluate the cytotoxicity of other lipid nanoparticles (e.g., cationic liposomes)[7].

IC50 & Target：Human Endogenous Metabolite

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References：

[1]. Sercombe L, et al. Advances and Challenges of Liposome Assisted Drug Delivery. Front Pharmacol. 2015 Dec 1;6:286.  [Content Brief]

[2]. Zelphati O, et al. Mechanism of oligonucleotide release from cationic liposomes. Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11493-8.  [Content Brief]

[3]. Bayburt T H, et al. Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins[J]. Nano letters, 2002, 2(8): 853-856.

[4]. Montal M, et al. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3561-6.  [Content Brief]

[5]. Lentz BR. Use of fluorescent probes to monitor molecular order and motions within liposome bilayers. Chem Phys Lipids. 1993 Sep;64(1-3):99-116.  [Content Brief]

[6]. Henzler Wildman KA, et al. Mechanism of lipid bilayer disruption by the human antimicrobial peptide, LL-37. Biochemistry. 2003 Jun 3;42(21):6545-58.  [Content Brief]

[7]. Albanese A, et al. The effect of nanoparticle size, shape, and surface chemistry on biological systems. Annu Rev Biomed Eng. 2012;14:1-16.  [Content Brief]