Knauer - Model Eurospher II Chiral NR - LC Columns
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Eurospher II Chiral NR 12 µm, Column 50x4 mm . Ultra pure, spherical high performance chiral HPLC phase based on silica gel with 100 A pores.
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Eurospher II Chiral NR is an immobilized brush-type phase with very broad generality and complementary selectivity to Eurospher II Chiral-AM and OM. It is but very stable because the Chiral-NR selector is covalently bound, so that you can use all HPLC-eluents. This phase is similar to the well-known immobilized IA, IB and IC columns.
The chiral selector, an aromatic compound, is covalently bound and has a similar broad chiral generality comparable with polysaccharide-derived chiral stationary phases. Eurospher II Chiral-NR is a brush-type phase with p-acceptor and p-donor groups, therefore the phase can be used for compounds which also contain p-acceptor and p-donor groups.
Eurospher II Chiral-NR-R shows the reversed elution order, because it is modified with the opposite enantiomeric chiral form compared to Eurospher II Chiral-NR.
Both phases are stable in all commonly used eluents, including aqueous systems. Typical eluents in the normal-phase mode are mixtures of Hexane and 2-Propanol, in the reversed phase mode mixtures of MeOH and water. The ideal operating pH-range is between 2 und 7, and the columns are stable up to 300 bar. Very often one can use the same or similar eluents like on the polysaccharide-derived CSPs.
Excellent for preparative applications
Due to the 100A° pores and the large surface area of 350 m2/g the phase has an excellent preparative capacity. With a 250 x 10 mm column one can separate 50 – 250 mg / injection and with a 250 x 20 mm column 250 – 1000 mg/ injection. In preparative separations it is an advantage to get the minor enantiomer eluting first.
- Brand Eurospher II
- Modification Chiral-NR
- Special characteristics immobilized brush type
- Base material silica gel
- Particle size 12 µm
- Pore size 100 Å
- Particle shape spherical, totally porous
- Length [mm] 50 mm
- Inner diameter [mm] 4 mm
- Hardware type Standard
- Connection 1/16'
- Application area chiral HPLC in NP mode
- Metal content < 10 ppm
- Recommended pH range 2 - 7
- Pressure rating 300 bar
- Temperature range up to 40°C (up to 50 °C short term)
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