Knauer - Model Eurospher II Chiral NR -LC Columns

Eurospher II Chiral NR is an immobilized brush-type phase with very broad generality and complementary selectivity to Eurospher II Chiral-AM and OM. It is but very stable because the Chiral-NR selector is covalently bound, so that you can use all HPLC-eluents. This phase is similar to the well-known immobilized IA, IB and IC columns.

The chiral selector, an aromatic compound, is covalently bound and has a similar broad chiral generality comparable with polysaccharide-derived chiral stationary phases. Eurospher II Chiral-NR is a brush-type phase with p-acceptor and p-donor groups, therefore the phase can be used for compounds which also contain p-acceptor and p-donor groups.

Eurospher II Chiral-NR-R shows the reversed elution order, because it is modified with the opposite enantiomeric chiral form compared to Eurospher II Chiral-NR.
Both phases are stable in all commonly used eluents, including aqueous systems. Typical eluents in the normal-phase mode are mixtures of Hexane and 2-Propanol, in the reversed phase mode mixtures of MeOH and water. The ideal operating pH-range is between 2 und 7, and the columns are stable up to 300 bar. Very often one can use the same or similar eluents like on the polysaccharide-derived CSPs.

Excellent for preparative applications

Due to the 100A° pores and the large surface area of 350 m2/g the phase has an excellent preparative capacity. With a 250 x 10 mm column one can separate 50 – 250 mg / injection and with a 250 x 20 mm column 250 – 1000 mg/ injection. In preparative separations it is an advantage to get the minor enantiomer eluting first.

• Brand Eurospher II
• Modification Chiral-NR
• Special characteristics immobilized brush type
• Base material silica gel
• Particle size 12 µm
• Pore size 100 Å
• Particle shape spherical, totally porous
• Length [mm] 50 mm
• Inner diameter [mm] 4 mm
• Hardware type Standard
• Connection 1/16"
• Application area chiral HPLC in NP mode
• Metal content < 10 ppm
• Recommended pH range 2 - 7
• Pressure rating 300 bar
• Temperature range up to 40°C (up to 50 °C short term)