Leadgene - Model 41604 -Bacteriophage T7 RNA Polymerase
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. This enzyme catalyzes the 3’ synthesis of RNA from DNA downstream from its promoter.
>98% as determined by SDS-PAGE.
Purified by Ni-NTA chromatography.
T7 RNA Polymerase is supplied in 100 mM Tris-HCl (pH 7.9), 20 mM KCl, 1 mM DTT, 1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) glycerol.
Stored at -20°C.
Avoid repeated freeze/thaw cycles.
Transcription reaction should be performed under RNase free condition. Use nuclease-free tubes, reagents, and water to avoid RNase contamination. Also, wear gloves when working with RNA.
Package
41601(50 U/μL) 5,000 U / 41602(50 U/μL) 25,000 U / 41603(50 U/μL) 50,000U / 41604(200 U/μL) 25,000 U
Unit Definition
One unit is defined as the amount of the enzyme incorporates 1 nmol of ATP into acid-insoluble product in 1 hour at 37°C.
Reaction Condition
1X RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
10X RNA Polymerase Reaction Buffer: 400 mM Tris-HCl (pH 8.0), 60 mM MgCl2, 100 mM DTT, and 20 mM spermidine.
Expression system
Escherichia coli