BioFire Defense

Life Science Melting Dyes and Kits

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Our roots go very deep into the life science and research industries with many of our first products being designed for PCR and Hi-Res Melting®. We continue to offer professional grade PCR master mixes along with our proprietary LCGreen® Plus melting dye.

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Our LightScanner Master Mix brilliantly provides a pre-optimized solution specifically designed to deliver superior PCR performance for Hi-Res Melting® and genotyping applications. Our master mix is compatible with all instructions featuring HRM capabilities. This master mix reduces time and saves money with easy-to-use premixed formulas. This secures downstream success by directly going from PCR to melting curve analysis and eliminates the risk of contamination.

Kit Details

This master mix incorporates a Taq polymerase, an antibody-based hot-start method, dNTPs, magnesium chloride, BioFire Defense’s proprietary LCGreen Plus dye and is formulated at a 2.5X concentration. The antibody-mediated hot-start method means no enzyme pre-activation is required before or during thermocycling, translating into much faster PCR protocol times than other methods of hot-start protection. The master mix includes a concentration of magnesium that is optimized to work for almost all reactions. Additional magnesium chloride solution is provided for further optimization if required. The master mix is also compatible with most additives used to enhance amplification efficiency of high GC amplicons.

Kit Contents

  • 2.5X Master Mix
  • 10 mM MgCI2
  • Reagent Grade Water
  • LightScanner Master Mix User’s Guide

Kit Part Nos.

  • HRLS-ASY-0002 – Reactions 100
  • HRLS-ASY-0003 – Reactions 500